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Bicarbonate (HCO₃⁻) concentration in serum/plasma

Published: August 9, 2026Updated: August 17, 2026

This test measures the amount of bicarbonate, a key chemical in your blood that helps regulate your body’s acid-base balance.

Quick summary

["Bicarbonate helps balance acidity in your blood, which is crucial for normal heart, kidney, and nerve function."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
94001-5
Short code
HCO3 1h p Vaso SerPl-sCnc
Synonyms
HCO3 1h p Vaso SerPl-sCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
22
Ref high
28
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Does Bicarbonate Measure?

Bicarbonate is a critical buffer in your blood, helping to maintain a stable pH (acidity level). It works alongside carbon dioxide and oxygen to keep your blood chemistry in balance. Abnormal levels can indicate conditions like metabolic acidosis or alkalosis, which may affect how your body processes nutrients or responds to stress.

Why Is This Test Done?

Your doctor may order this test if you have symptoms like confusion, rapid breathing, fatigue, or muscle cramps. It’s also used to diagnose or monitor conditions such as kidney disease, diabetes, or heart problems. Sometimes, it’s checked alongside other blood tests to assess overall health or response to treatments.

How Is the Test Performed?

A small blood sample is taken from a vein, usually in your arm. The sample is sent to a lab where it’s analyzed to measure bicarbonate levels. No special preparation is needed, but you may be asked to fast or avoid certain medications before the test, depending on your doctor’s instructions.

What Are Possible Results?

Results are typically reported as a numerical value (e.g., mmol/L) but aren’t always provided with a specific range. Your doctor will compare your result to your body’s normal balance and consider your symptoms and medical history to interpret it.

Reference range

NCBI: HCO3- 22-28 mmol/LN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Serum bicarbonate level

Frequently asked questions

What Does a Low Bicarbonate Level Mean?
A low bicarbonate level may mean your body is producing too much acid or losing too much bicarbonate. This can happen with kidney problems, severe dehydration, or certain medications. Your doctor may recommend lifestyle changes, medications, or further tests to find the cause.
What Does a High Bicarbonate Level Mean?
High bicarbonate levels can occur if you’ve lost too much stomach acid (e.g., from vomiting) or if your body is retaining too much bicarbonate (e.g., from certain medications). This can sometimes be a sign of overuse of antacids or other conditions affecting your kidneys or lungs. Your doctor will work with you to address the underlying issue.
How Often Should I Get This Test?
The frequency depends on your health condition. If you have a chronic illness like kidney disease or diabetes, your doctor may order this test regularly. If you’re healthy, it’s usually only done when needed for diagnosis or monitoring a specific concern.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy