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Bicarbonate concentration in blood (serum/plasma)

Published: August 9, 2026Updated: August 17, 2026

This test measures the amount of bicarbonate, a key chemical in your blood that helps balance acidity and alkalinity.

Quick summary

["Bicarbonate helps regulate your body’s pH and supports normal blood pressure and metabolism."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
53086-5
Short code
HCO3 Fld.NB-sCnc
Synonyms
HCO3 Fld.NB-sCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
22
Ref high
28
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Does This Test Measure?

Bicarbonate is a critical buffer in your blood, helping maintain a stable pH level. Your body produces bicarbonate naturally, and it plays a role in how your kidneys and lungs regulate acid-base balance. Abnormal levels can indicate conditions affecting your metabolism, kidney function, or breathing.

Why Might You Need This Test?

Doctors may order this test if you have symptoms like fatigue, confusion, shortness of breath, or muscle cramps. It’s also used to diagnose or monitor conditions such as metabolic acidosis or alkalosis, kidney disease, or respiratory disorders.

How Is the Test Done?

A small blood sample is drawn from a vein in your arm. The sample is sent to a lab where it’s analyzed to measure bicarbonate levels. No special preparation is needed, but fasting may be required depending on your specific test instructions.

What Are Possible Results?

Results are typically reported in milliequivalents per liter (mEq/L). While reference ranges vary by lab, low levels may suggest metabolic acidosis, while high levels could indicate metabolic alkalosis or other conditions.

Reference range

NCBI: HCO3- 22-28 mmol/LN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Bicarbonate (HCO₃⁻) level

Frequently asked questions

What could cause abnormal bicarbonate levels?
Low bicarbonate levels (acidosis) can occur due to conditions like kidney failure, severe diarrhea, or uncontrolled diabetes. High bicarbonate levels (alkalosis) may result from vomiting, excessive antacid use, or overbreathing (hyperventilation). Chronic conditions like chronic obstructive pulmonary disease (COPD) or kidney disease can also affect bicarbonate levels over time.
How long will it take to get my results?
Results are usually available within 24 to 48 hours, depending on your lab’s processing time. Your healthcare provider will discuss the results with you during your follow-up visit.
Do I need to prepare for this test?
Most often, no special preparation is needed. However, your doctor may ask you to avoid certain foods, medications, or activities for a few days before the test. Always follow your healthcare provider’s instructions.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy