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Bicarbonate (HCO₃⁻) Level in Serum

Published: August 9, 2026Updated: August 17, 2026

This test measures the amount of bicarbonate, a key chemical in your blood that helps balance acidity and alkalinity. It’s often checked when doctors suspect conditions like metabolic acidosis or alkalosis.

Quick summary

["Bicarbonate levels help diagnose and monitor acid-base imbalances in your blood."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
39459-3
Short code
HCO3 std BldCoV-sCnc
Synonyms
HCO3 std BldCoV-sCnc
Category
chemistry
Sample
whole-blood
Container
Lavender-top (EDTA)
Units
N/A
Ref low
22
Ref high
28
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
3 mL
Stability
Room temperature: 4h, Refrigerated: 24h
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Matters

Bicarbonate is a critical buffer in your blood, helping maintain a stable pH (acidity level) that keeps your body functioning properly. When levels are too high or too low, it can signal underlying health issues, such as kidney problems, diabetes, or respiratory conditions. Your doctor may order this test to check for metabolic disorders or to assess how well your body is handling acid-base balance after treatments like dialysis or medications.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to fast for a few hours before the blood draw if your doctor specifies. On the day of the test, avoid smoking or drinking caffeine, as these can temporarily affect your blood chemistry. Wear a loose shirt so your doctor can easily access your arm for the blood sample.

What Happens During the Test

A healthcare professional will clean your arm with an antiseptic and insert a small needle to draw blood from a vein. The process is quick and typically causes only a minor pinch. The blood sample is then sent to a lab for analysis. You can usually return to your normal activities immediately after.

Possible Results and Next Steps

Your results will be compared to standard reference ranges, though exact values may vary by lab. If your bicarbonate level is outside the normal range, your doctor may recommend further testing or treatments to address the underlying cause, such as adjusting medications or treating dehydration, kidney disease, or other conditions. Follow-up visits may be scheduled to monitor progress.

Reference range

NCBI: HCO3- 22-28 mmol/LN/A

Specimen: whole-blood

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Serum Bicarbonate

Frequently asked questions

What causes bicarbonate levels to be too high or too low?
High bicarbonate levels (metabolic alkalosis) can occur due to conditions like vomiting, diarrhea, excessive use of antacids, or kidney problems that cause the body to retain bicarbonate. Low bicarbonate levels (metabolic acidosis) may result from kidney disease, diabetic ketoacidosis, severe dehydration, or certain medications. Your doctor will consider your full medical history and other test results to determine the cause.
Can lifestyle changes affect bicarbonate levels?
Yes, certain lifestyle factors can influence bicarbonate levels. For example, excessive vomiting or diarrhea can lead to alkalosis, while chronic dehydration or poor kidney function can contribute to acidosis. Avoiding excessive antacid use, staying hydrated, and managing chronic conditions like diabetes or kidney disease can help support balanced bicarbonate levels.
How often should I get this test?
The frequency of this test depends on your health condition. If you have a chronic illness like kidney disease or diabetes, your doctor may order it regularly to monitor your progress. If you’re asymptomatic but have risk factors (e.g., frequent vomiting, kidney issues), your doctor might recommend periodic checks. Always follow your healthcare provider’s guidance.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy