Bicarbonate (HCO₃⁻) Level in Serum
This test measures the amount of bicarbonate, a key chemical in your blood that helps balance acidity and alkalinity. It’s often checked when doctors suspect conditions like metabolic acidosis or alkalosis.
["Bicarbonate levels help diagnose and monitor acid-base imbalances in your blood."]
Key facts at a glance
- LOINC
- 39459-3
- Short code
- HCO3 std BldCoV-sCnc
- Synonyms
- HCO3 std BldCoV-sCnc
- Category
- chemistry
- Sample
- whole-blood
- Container
- Lavender-top (EDTA)
- Units
- N/A
- Ref low
- 22
- Ref high
- 28
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Specimen volume
- 3 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h
- Hemolysis max
- 0
- Lipemia max
- 0
- Icterus max
- 0
- TAT
- 4–24 hours
- Order
- routine
Why This Test Matters
Bicarbonate is a critical buffer in your blood, helping maintain a stable pH (acidity level) that keeps your body functioning properly. When levels are too high or too low, it can signal underlying health issues, such as kidney problems, diabetes, or respiratory conditions. Your doctor may order this test to check for metabolic disorders or to assess how well your body is handling acid-base balance after treatments like dialysis or medications.
What to Expect Before the Test
No special preparation is usually needed, but you may be asked to fast for a few hours before the blood draw if your doctor specifies. On the day of the test, avoid smoking or drinking caffeine, as these can temporarily affect your blood chemistry. Wear a loose shirt so your doctor can easily access your arm for the blood sample.
What Happens During the Test
A healthcare professional will clean your arm with an antiseptic and insert a small needle to draw blood from a vein. The process is quick and typically causes only a minor pinch. The blood sample is then sent to a lab for analysis. You can usually return to your normal activities immediately after.
Possible Results and Next Steps
Your results will be compared to standard reference ranges, though exact values may vary by lab. If your bicarbonate level is outside the normal range, your doctor may recommend further testing or treatments to address the underlying cause, such as adjusting medications or treating dehydration, kidney disease, or other conditions. Follow-up visits may be scheduled to monitor progress.
Reference range
NCBI: HCO3- 22-28 mmol/LN/A
No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
◆Associated findings1
Short name
Serum Bicarbonate
Frequently asked questions
What causes bicarbonate levels to be too high or too low?
Can lifestyle changes affect bicarbonate levels?
How often should I get this test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy