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Bicarbonate concentration in whole blood (electrolyte measurement)

Published: August 9, 2026Updated: August 17, 2026

This test measures the amount of bicarbonate, a key chemical in your blood that helps regulate your body’s acid-base balance.

Quick summary

["Bicarbonate helps maintain your blood’s pH level, ensuring proper function of organs and tissues."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
12513-8
Short code
HCO3 Wat-sCnc
Synonyms
HCO3 Wat-sCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
22
Ref high
28
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Does This Test Measure?

Bicarbonate is a critical buffer in your blood, helping to neutralize acids and maintain a stable pH. Your body tightly controls bicarbonate levels to prevent conditions like acidosis or alkalosis, which can affect how your heart, lungs, and other organs work. This test is often used alongside other blood chemistry tests to assess overall acid-base balance.

Why Might You Need This Test?

Doctors may order this test if you have symptoms like shortness of breath, fatigue, confusion, or muscle weakness—common signs of imbalanced acid-base levels. It’s also used in monitoring conditions like kidney disease, diabetes, or severe dehydration, where bicarbonate levels can change.

How Is the Test Performed?

A small blood sample is drawn from a vein, usually in your arm. The sample is sent to a lab where it’s analyzed to measure bicarbonate concentration. No special preparation is needed, but fasting may be required depending on your specific test order.

What Are Common Results?

Results are reported as a concentration value (typically in millimoles per liter, mmol/L). While exact ranges vary by lab, low bicarbonate may indicate acidosis, while high levels could suggest alkalosis. Your doctor will interpret results in the context of your overall health.

Reference range

NCBI: HCO3- 22-28 mmol/LN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Whole Blood Bicarbonate

Frequently asked questions

What causes abnormal bicarbonate levels?
Abnormal bicarbonate levels can result from conditions affecting your lungs (e.g., chronic obstructive pulmonary disease), kidneys (e.g., kidney failure), or metabolic processes (e.g., diabetes, severe dehydration). Chronic conditions like heart disease or liver problems may also influence bicarbonate.
How often should I get this test?
Your doctor will determine how often you need this test based on your health status, symptoms, and any ongoing conditions. Regular monitoring may be recommended if you have chronic illnesses or take medications that affect acid-base balance.
Can I prepare for this test?
Mostly no—just rest assured the test is quick and painless. However, follow any specific instructions your doctor provides, such as fasting or avoiding certain medications, to ensure accurate results.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy