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Hemoglobin concentration in whole blood (autoanalyzer method)

Published: August 9, 2026Updated: August 17, 2026

This test measures the amount of hemoglobin in your blood, which carries oxygen to your body’s tissues. Hemoglobin levels help doctors assess whether you have enough red blood cells to stay healthy.

Quick summary

["Hemoglobin levels indicate your body’s ability to deliver oxygen effectively."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
71833-8
Short code
Hct VFr.DF Bld Auto
Synonyms
Hct VFr.DF Bld Auto
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
36
Ref high
50
Critical range
18 N/A – 60 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
automated-count
CPT
85014
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why is this test done?

Your healthcare provider may order this test to check for anemia (low red blood cells), polycythemia (too many red blood cells), or other blood-related conditions. It’s also used during pregnancy, before surgeries, or if you have symptoms like fatigue, shortness of breath, or pale skin.

What does a low or high hemoglobin level mean?

Low hemoglobin may signal anemia, iron deficiency, or blood loss, while high levels could indicate conditions like dehydration, polycythemia, or certain lung diseases. Your results will be compared to standard ranges for your age and sex.

How is this test performed?

A small blood sample is drawn from a vein in your arm. The blood is then analyzed automatically to measure hemoglobin concentration, which is reported as a percentage or grams per deciliter (g/dL).

What do my results mean?

Your results will be reviewed by your healthcare provider alongside other tests and your medical history. They’ll help determine if further testing or treatment is needed, such as adjusting medications or addressing underlying health issues.

Reference range

Adult males: 38-50%; Females: 36-44%N/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical significance: Hemoglobin is critical for oxygen transport. Abnormal levels can signal systemic health risks, including chronic diseases, infections, or nutritional deficiencies. Regular monitoring is especially important for patients with conditions like heart disease, kidney problems, or those undergoing chemotherapy.

Short name

Hemoglobin (Hb) Level

Frequently asked questions

What causes hemoglobin levels to change?
Hemoglobin can drop due to blood loss (e.g., from injuries or heavy periods), poor nutrition (e.g., low iron or vitamin B12), or chronic illnesses like kidney disease or liver problems. It may rise from conditions like dehydration, high altitude, or certain cancers.
How often should I get this test?
Frequency depends on your health status. Routine checks may occur annually or more often if you have a condition requiring close monitoring, such as anemia or heart disease. Your doctor will guide you on timing.
Can lifestyle changes affect hemoglobin levels?
Yes! Eating iron-rich foods (like lean meats, spinach, or fortified cereals), staying hydrated, and avoiding smoking can help maintain stable levels. If you suspect dietary deficiencies, discuss supplements with your provider.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy