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Homocysteine (total) by high-performance liquid chromatography (HPLC) or related mass spectrometry

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of homocysteine, a type of amino acid, in your blood. High levels may increase your risk of certain health problems.

Quick summary

["Homocysteine levels can help assess risk for heart disease and other conditions."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
13399-1
Short code
(Hcys)2 Amn-mCnc
Synonyms
(Hcys)2 Amn-mCnc
Category
chemistry
Sample
fluid
Container
Sterile tube
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
2-5 mL
Stability
Room temperature: 2h, Refrigerated: 24h
Hemolysis max
300
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you have a family history of heart disease, stroke, or blood vessel problems. It may also be done to monitor conditions like kidney disease, certain vitamins deficiencies, or if you’re taking medications that affect homocysteine levels.

What to Expect Before the Test

No special preparation is needed. You may have a small needle inserted into your arm to draw blood. If you’re on certain medications (like folic acid, vitamin B6, or B12), let your provider know, as they may affect results.

What the Results Mean

Results are compared to general reference ranges, but individual ranges can vary. High levels may signal increased risk for vascular issues, while low levels may indicate deficiencies in certain vitamins.

Common Causes of Abnormal Results

High homocysteine levels can be caused by genetics, kidney disease, vitamin deficiencies (like folate, B6, or B12), smoking, or certain medications. Low levels may occur with vitamin B deficiencies.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Homocysteine Level

Frequently asked questions

How often should I get this test?
Your provider will determine frequency based on your health status, risk factors, and other tests. Regular monitoring may be recommended if you have conditions like heart disease or kidney problems.
Can I eat or drink before the test?
You can eat and drink normally unless instructed otherwise. Avoid alcohol for 24 hours before testing, as it may affect results.
Does this test interact with any medications?
Certain medications (like folic acid supplements or some blood pressure drugs) can influence homocysteine levels. Always inform your provider about all medications you’re taking.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy