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HDL Cholesterol, Serum/Plasma — Mass Concentration

Published: August 9, 2026Updated: August 16, 2026

Measures high-density lipoprotein cholesterol in blood to help assess cardiovascular risk.

Quick summary

["HDL cholesterol is often called “good” cholesterol, but it should be interpreted with the rest of your lipid profile."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
47221-7
Short code
HDL1+2c SerPl-mCnc
Synonyms
HDL1+2c SerPl-mCnc
Category
lipid
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
40
Ref high
60
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83700
SNOMED
113079008
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This blood test measures the amount of cholesterol carried by high-density lipoprotein (HDL). HDL helps transport cholesterol away from artery walls back to the liver.

Why might I need this test?

HDL cholesterol is usually part of a lipid profile or cholesterol panel. Your clinician may use it along with total cholesterol, LDL cholesterol, triglycerides, and your medical history to assess cardiovascular risk.

How is the test performed?

A small blood sample is drawn from a vein and analyzed in a laboratory. It is commonly collected at the same time as the other tests in a lipid panel.

How do I prepare for the test?

Fasting is not always required, but follow your clinician’s instructions, especially if you are also having LDL cholesterol or triglycerides measured. Tell your clinician about cholesterol-lowering medicines or supplements you take.

Reference range

Adult males: >40 mg/dL; Females: >50 mg/dLN/A

Specimen: serum

Fasting for 12-14 hours preferred. Water is permitted. Avoid alcohol for 24 hours before the test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

HDL cholesterol

Frequently asked questions

Is HDL cholesterol the same as “good cholesterol”?
Yes. HDL is commonly called “good cholesterol” because it helps move cholesterol away from artery walls. Its level is only one part of overall cardiovascular risk.
Do I need to fast for this test?
Fasting may be requested when HDL is measured with a full lipid panel, but it is not required for every HDL test. Follow the preparation instructions provided by your clinician or laboratory.
Can medicines or supplements affect HDL cholesterol?
Yes. Cholesterol-lowering medicines, some other medicines, diet, weight changes, and physical activity can affect the result. Do not stop prescribed medicines without talking with your clinician.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy