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HDL Alpha-3, Serum — Substance Concentration

Published: August 9, 2026Updated: August 16, 2026

Measures the concentration of HDL alpha-3, a subfraction of high-density lipoprotein, in a serum sample.

Quick summary

["This test measures a component of HDL cholesterol in the blood."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
86222-7
Short code
HDL alpha 3 Ser-sCnc
Synonyms
HDL alpha 3 Ser-sCnc
Category
liver
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
44
Ref high
147
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
84075
SNOMED
27550007
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This blood test measures the concentration of HDL alpha-3 in serum. HDL alpha-3 is a subfraction of high-density lipoprotein (HDL), the particle that carries cholesterol away from tissues back to the liver.

Why might I need this test?

A clinician may order this test as part of an evaluation of cholesterol metabolism or when other lipid measurements need more detailed interpretation. It is usually considered alongside total cholesterol, HDL cholesterol, LDL cholesterol, and triglycerides.

How is the test performed?

A blood sample is drawn, and the serum portion is analyzed in the laboratory to determine the concentration of HDL alpha-3. The result is reported as a concentration in the blood.

How do I prepare for the test?

No special preparation is usually required unless your clinician gives different instructions. Because food can affect lipid measurements, tell the laboratory if you ate recently and follow any fasting directions provided.

Reference range

Adult: 44-147 U/LN/A

Specimen: serum

Fasting for 12-14 hours preferred. Water is permitted. Avoid alcohol for 24 hours before the test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

HDL Alpha-3

Frequently asked questions

Is HDL alpha-3 the same as HDL cholesterol?
No. HDL cholesterol measures the cholesterol carried within all HDL particles, while this test measures a specific HDL subfraction called alpha-3.
Does a low or high result mean I have heart disease?
Not by itself. HDL alpha-3 is interpreted with the rest of your lipid profile and other risk factors to assess cholesterol metabolism and cardiovascular risk.
Do I need to fast before this test?
Fasting may be requested when the test is part of a complete lipid profile, but it is not always required. Follow the preparation instructions provided by your clinician or laboratory.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy