Cholesterol in HDL-3 Beta, Serum/Plasma — Electrophoresis, Mass Fraction
A specialized lipid test measuring cholesterol in the HDL-3 beta subfraction of HDL, commonly called “good cholesterol,” in serum or plasma.
["This test measures a specific component of HDL cholesterol, but its result is most useful when interpreted with the rest of your lipid profile."]
Key facts at a glance
- LOINC
- 54470-0
- Short code
- HDLc 3b MFr SerPl Elph
- Synonyms
- HDLc 3b MFr SerPl Elph
- Category
- lipid
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Ref low
- 40
- Ref high
- 60
- Critical range
- 7.2 N/A – 7.6 N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- electrophoresis
- CPT
- 83700
- SNOMED
- 113079008
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
What is this test?
This test measures the amount of cholesterol in a specific part of high-density lipoprotein (HDL), known as the HDL-3 beta subfraction. HDL is often called “good cholesterol” because it helps transport cholesterol away from the arteries.
Why might I need this test?
It may be ordered as part of an in-depth evaluation of cholesterol levels or cardiovascular risk. It can also be used in specialized research or when a clinician wants more detail about the distribution of HDL subfractions.
How is the test performed?
A blood sample is collected and separated into serum or plasma. The laboratory uses electrophoresis to separate the HDL subfractions and then measures the cholesterol within the HDL-3 beta fraction.
How do I prepare for the test?
No special preparation is universally required, although fasting or avoiding alcohol for a period may be requested for some lipid tests. Follow the laboratory’s instructions, and do not stop prescribed medication unless your clinician tells you to.
Reference range
Adult males: >40 mg/dL; Females: >50 mg/dLN/A
Fasting for 12-14 hours preferred. Water is permitted. Avoid alcohol for 24 hours before the test.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
HDL-3 beta is one of several HDL subfractions, and the independent clinical meaning of this measurement is not as well established as it is for total HDL cholesterol. An abnormal result may reflect differences in cholesterol metabolism, metabolic conditions, or inherited lipid patterns, but it does not diagnose a specific disorder by itself. The result should be interpreted with total cholesterol, HDL cholesterol, triglycerides, LDL cholesterol, and your overall health history.
Short name
HDL-3 beta cholesterol
Frequently asked questions
Is HDL-3 beta the same as regular HDL cholesterol?
Does a high result mean I am healthier?
Do I need to fast before this test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy