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HDL Cholesterol, Serum/Plasma — Electrophoresis, Arbitrary Concentration

Published: August 9, 2026Updated: August 16, 2026

An electrophoresis-based test that analyzes HDL cholesterol in serum or plasma and may report HDL particle subfractions or an electrophoresis-specific concentration.

Quick summary

["This test characterizes HDL cholesterol and may provide additional information about the distribution of HDL particles in the blood."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
12772-0
Short code
HDLc SerPl Elph-aCnc
Synonyms
HDLc SerPl Elph-aCnc
Category
lipid
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
40
Ref high
60
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
electrophoresis
CPT
83700
SNOMED
113079008
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

High-density lipoprotein (HDL) is a group of cholesterol-carrying particles in the blood. This test uses electrophoresis to analyze HDL cholesterol in a serum or plasma sample. Depending on the laboratory, the report may show HDL subfractions or a method-specific concentration rather than the standard direct HDL cholesterol result.

Why might I need this test?

This test may be ordered as part of a lipid evaluation or when a clinician wants more detailed information about HDL particle composition. It may also be used in specialized cardiovascular risk assessment, although routine risk assessment generally uses the standard HDL cholesterol result from a lipid panel.

How is the test performed?

A blood sample is collected, and the serum or plasma is analyzed by electrophoresis. The laboratory separates and measures the HDL cholesterol components, then reports the concentration or relative distribution according to its validated method.

How do I prepare for the test?

Follow the preparation instructions provided by the laboratory or ordering clinician. Some lipid tests are performed fasting, but this electrophoresis test may not always require fasting. Do not stop prescribed medications unless your clinician specifically instructs you to do so.

Reference range

Adult males: >40 mg/dL; Females: >50 mg/dLN/A

Specimen: serum

Fasting for 12-14 hours preferred. Water is permitted. Avoid alcohol for 24 hours before the test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

HDL Cholesterol Electrophoresis

Frequently asked questions

Is this the same as the HDL cholesterol result on a routine lipid panel?
Not necessarily. A routine lipid panel usually reports a standard HDL cholesterol concentration, while this test uses electrophoresis and may provide additional particle or subfraction information.
Does the test measure all the cholesterol in my blood?
No. It specifically analyzes cholesterol associated with HDL rather than total cholesterol or every cholesterol-containing particle.
Why do laboratories report this test in different units?
Electrophoresis methods may report relative distributions or method-specific concentrations rather than the familiar mass-per-volume units used for standard HDL cholesterol. The report should identify the units used.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy