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High-Density Lipoprotein Cholesterol, Serum/Plasma — Electrophoresis, Mass Concentration

Published: August 9, 2026Updated: August 16, 2026

Measures the amount of cholesterol carried by high-density lipoprotein (HDL) in blood using electrophoretic separation.

Quick summary

["HDL cholesterol is one part of the blood lipid panel and is interpreted together with total cholesterol, LDL cholesterol, and triglycerides."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
49130-8
Short code
HDLc SerPl Elph-mCnc
Synonyms
HDLc SerPl Elph-mCnc
Category
lipid
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
40
Ref high
60
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
electrophoresis
CPT
83700
SNOMED
113079008
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This test measures the concentration of cholesterol in high-density lipoprotein (HDL) particles in serum or plasma. HDL is a lipoprotein that transports cholesterol in the blood.

Why might I need this test?

HDL cholesterol is usually assessed as part of a lipid panel to help evaluate cardiovascular risk. It may also be checked when monitoring treatment for high cholesterol or when an unusually high or low result is suspected.

How is the test performed?

A blood sample is separated into its liquid fraction, and the lipoproteins are distinguished by electrophoresis. The amount of cholesterol associated with the HDL fraction is then measured and reported as a mass concentration.

How do I prepare for the test?

Follow the instructions provided by your healthcare professional or laboratory. Fasting may be requested for lipid testing, but it is not always required, especially when the test is ordered with other blood tests.

Reference range

Adult males: >40 mg/dL; Females: >50 mg/dLN/A

Specimen: serum

Fasting for 12-14 hours preferred. Water is permitted. Avoid alcohol for 24 hours before the test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

HDL cholesterol

Frequently asked questions

Is a high HDL cholesterol result always good?
Higher HDL cholesterol is generally considered favorable, but it does not fully protect against heart disease or stroke. The overall lipid profile and cardiovascular risk factors remain important.
What can cause low HDL cholesterol?
Low HDL cholesterol can be associated with lifestyle factors, metabolic conditions, medications, and genetic conditions. Your healthcare professional can help interpret the result in your individual context.
Do I need to fast before this test?
Fasting may be recommended depending on the laboratory’s protocol and the reason for testing. Follow the specific preparation instructions provided with the test order.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy