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HDL Cholesterol, Serum/Plasma — Qualitative

Published: August 9, 2026Updated: August 16, 2026

This blood test evaluates high-density lipoprotein (HDL), often called “good” cholesterol, to help assess cardiovascular risk.

Quick summary

["HDL cholesterol helps move cholesterol away from artery walls, but it is interpreted along with other cholesterol measurements and your health history."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
27340-9
Short code
HDLc SerPl Ql
Synonyms
HDLc SerPl Ql
Category
lipid
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
40
Ref high
60
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83700
SNOMED
113079008
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This test measures HDL cholesterol in serum or plasma. HDL is a type of cholesterol-carrying protein that helps transport cholesterol from tissues back to the liver.

Why might I need this test?

It may be ordered as part of a cholesterol panel or to help assess your risk of heart disease and stroke. Your clinician will consider it together with LDL cholesterol, total cholesterol, triglycerides, and other risk factors.

How is the test performed?

A blood sample is drawn and separated into serum or plasma in the laboratory. The laboratory then analyzes the sample to determine the HDL cholesterol result.

How do I prepare for the test?

Fasting is usually not required for a routine HDL cholesterol test, but follow your clinician’s instructions. If you are also having triglycerides or other fasting lipid tests, you may be asked to fast for 9 to 12 hours beforehand.

Reference range

Adult males: >40 mg/dL; Females: >50 mg/dLN/A

Specimen: serum

Fasting for 12-14 hours preferred. Water is permitted. Avoid alcohol for 24 hours before the test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

HDL cholesterol

Frequently asked questions

What does HDL stand for?
HDL stands for high-density lipoprotein, a particle that carries cholesterol through the blood. It is often called “good” cholesterol because it helps move cholesterol away from artery walls.
Does this test require fasting?
Fasting is usually not needed for HDL testing by itself. Your clinician may request fasting if triglycerides or another test requiring a fasting sample are being checked at the same time.
Can HDL cholesterol alone determine my heart disease risk?
No. HDL is one part of cardiovascular risk assessment and is interpreted with LDL cholesterol, total cholesterol, triglycerides, blood pressure, smoking status, and other personal health factors.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy