Line Blot Immunoassay for Antibodies to Borrelia burgdorferi (Lyme Disease Antibody Screening)
This test detects antibodies in your blood that may indicate exposure to *Borrelia burgdorferi*, the bacterium responsible for Lyme disease, often spread through tick bites.
["A simple blood test to check for Lyme disease antibodies, helping identify possible exposure."]
Key facts at a glance
- LOINC
- 101218-6
- Short code
- H dobrava IgM SerPl Ql Line blot
- Synonyms
- H dobrava IgM SerPl Ql Line blot
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- line-blot
- CPT
- 82784
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
What This Test Measures
The line blot immunoassay identifies specific antibodies your immune system produces in response to *Borrelia burgdorferi*. If antibodies are present, it suggests past or current infection. This test uses a qualitative method to detect multiple related antigens from the bacterium.
How the Test Works
Your blood sample is tested on a membrane with specific antigens. If antibodies bind to these antigens, they create visible lines, confirming potential exposure. This method is quick and often used as an initial screening tool.
When You Might Need This Test
Your healthcare provider may recommend this test if you have symptoms like fever, fatigue, joint pain, or a rash (e.g., bull’s-eye rash) after a tick bite, especially in areas where Lyme disease is common.
What to Expect Before the Test
No special preparation is needed. You’ll have a simple blood draw, usually from your arm, and the results are typically available within a few days.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Positive results suggest possible Lyme disease exposure but do not confirm infection alone. Follow-up testing (e.g., ELISA or Western blot) may be needed for diagnosis. Early detection is important for treatment, which can help prevent long-term complications.
Short name
Lyme Disease Antibody Screen (Line Blot)
Frequently asked questions
Why was this test ordered?
What does a positive result mean?
Do I need to take any special steps if my results are positive?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy