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Anti-nuclear antibody (ANA) by immunofluorescence (serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures antibodies in your blood that may indicate certain autoimmune conditions, such as lupus or rheumatoid arthritis. It uses a special fluorescent dye to detect these antibodies on prepared tissue samples.

Quick summary

["A simple blood test to check for autoimmune antibodies that could signal underlying inflammation or connective tissue disorders."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
5213-4
Short code
Heteroph Ab Ser Ql LA
Synonyms
Heteroph Ab Ser Ql LA
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
la
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may recommend this test if you have symptoms like joint pain, fatigue, rash, or unexplained fever, or if you have a family history of autoimmune diseases. It’s also used as part of routine monitoring for known conditions like lupus or scleroderma.

What to Expect During the Test

You’ll have a small needle inserted into your arm to draw blood. The process takes about 5 minutes. The sample is then sent to a lab where it’s analyzed for specific antibodies using a fluorescent microscope.

What the Results Mean

Results are reported as a positive or negative finding, not a specific number. A positive result means antibodies were detected, which may prompt further testing to identify the specific type of autoimmune disease.

Common Conditions Linked to Positive Results

A positive ANA test is common in autoimmune diseases like systemic lupus erythematosus (lupus), Sjögren’s syndrome, or mixed connective tissue disease. However, some healthy people also test positive, so additional tests are needed for diagnosis.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

ANA Screen

Frequently asked questions

What does a positive ANA test mean for my health?
A positive ANA test alone doesn’t diagnose an autoimmune disease. It’s often used as a screening tool. Follow-up tests, such as specific antibody panels or clinical correlation, are needed to determine if you have a specific condition.
How often should I get this test?
Your provider will determine how often you need retesting based on your symptoms, medical history, and other test results. Some people with chronic conditions may need regular monitoring.
Can lifestyle changes affect this test?
While certain medications (like steroids) or infections can temporarily alter test results, lifestyle factors like diet or stress do not directly impact ANA antibody levels. Always discuss any changes with your healthcare team.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy