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Anti-nuclear antibody (ANA) by immunofluorescence

Published: August 9, 2026Updated: August 16, 2026

This test measures antibodies against nuclear components in your blood, often used to help diagnose autoimmune conditions.

Quick summary

["Helps identify autoimmune diseases by detecting abnormal antibodies in your blood."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
31419-5
Short code
Heteroph Ab Snv Ql
Synonyms
Heteroph Ab Snv Ql
Category
chemistry
Sample
fluid
Container
Sterile tube
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
2-5 mL
Stability
Room temperature: 2h, Refrigerated: 24h
Hemolysis max
300
TAT
4–24 hours
Order
routine

What Is an ANA Test Used For?

ANA tests are primarily used to screen for autoimmune diseases, such as lupus, rheumatoid arthritis, or other connective tissue disorders. They don’t confirm a diagnosis alone but may prompt further testing if results are positive.

How Is the ANA Test Performed?

A small blood sample is drawn from your arm. The serum is then tested using immunofluorescence to detect specific antibodies that target nuclear parts of cells.

What Are Common Causes of Positive ANA Results?

Positive ANA results can occur in autoimmune diseases, infections, certain medications, or during pregnancy. False positives may also happen due to normal variations in antibody levels.

When Might You Need This Test?

Your doctor may recommend an ANA test if you have symptoms like joint pain, fatigue, rash, or unexplained fever, or if you have a family history of autoimmune diseases.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

ANA (IF)

Frequently asked questions

Why do I need an ANA test if I don’t have symptoms?
Your doctor may order it as a screening test, especially if you have risk factors (e.g., family history, certain occupations, or chronic illnesses). It helps monitor conditions like lupus or rheumatoid arthritis over time.
Can lifestyle changes affect ANA test results?
Lifestyle factors like diet, stress, or infections may influence antibody levels temporarily, but they don’t change the test’s clinical significance. Results should be interpreted alongside your overall health.
What if my ANA test is positive but I don’t have symptoms?
A positive ANA doesn’t always mean you have an autoimmune disease. Follow-up testing and medical evaluation are needed to determine the cause and next steps.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy