Serum Protein Electrophoresis (Bromocresol Purple Method)
This test measures the distribution of different protein fractions in your blood serum using electrophoresis with a bromocresol purple dye. It helps identify abnormalities in protein composition, which may suggest conditions like multiple myeloma, liver disease, or other systemic disorders.
["This test helps doctors diagnose and monitor conditions affecting protein balance in your blood."]
Key facts at a glance
- LOINC
- 4556-7
- Short code
- Hgb Abruz Bld Ql Elph Acid
- Synonyms
- Hgb Abruz Bld Ql Elph Acid
- Category
- hematology
- Sample
- whole-blood
- Container
- Lavender-top (EDTA)
- Units
- N/A
- Ref low
- 12
- Ref high
- 17.5
- Critical range
- 7 N/A – 20 N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- electrophoresis-ph
- CPT
- 85018
- SNOMED
- 900000000000003004
- Specimen volume
- 3 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h
- Hemolysis max
- 0
- Lipemia max
- 0
- Icterus max
- 0
- TAT
- 4–24 hours
- Order
- routine
What Proteins Does This Test Measure?
Electrophoresis separates blood proteins into several bands based on their size and electrical charge. Common protein fractions detected include albumin, alpha-1 globulins, alpha-2 globulins, beta globulins, and gamma globulins (such as immunoglobulins). Abnormal patterns may indicate underlying health issues.
Why Might You Need This Test?
Your doctor may order this test if you have symptoms like fatigue, unexplained weight loss, swelling, or if they suspect conditions like multiple myeloma, liver disease, kidney disease, or chronic inflammation. It’s also used to monitor treatment progress for certain disorders.
Sample Collection and Preparation
A blood sample is drawn from a vein, typically in your arm. The blood is then processed to separate serum (the liquid part without clots) for testing. The bromocresol purple dye helps visualize protein bands during electrophoresis.
What to Expect Before the Test
No special preparation is usually needed, but you may be asked to avoid certain medications or foods that could interfere with results. For example, some medications or dietary supplements might affect protein levels.
Reference range
Adult males: 13.5-17.5 g/dL; Females: 12.0-16.0 g/dLN/A
No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Clinical Significance: How This Test Helps Healthcare Providers
Short name
Protein Electrophoresis
Frequently asked questions
How can I prepare for this test?
What are the typical reference ranges for protein electrophoresis?
Can this test detect early signs of diseases like cancer or liver disease?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy