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Hemoglobin Mass Fraction by Flow Cytometry (Red Blood Cell Analysis)

Published: August 9, 2026Updated: August 16, 2026

This test measures the proportion of hemoglobin within your red blood cells using flow cytometry. It helps assess red blood cell health and potential abnormalities in hemoglobin distribution.

Quick summary

["This test helps evaluate red blood cell function and identify conditions affecting hemoglobin levels."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
71876-7
Short code
Hgb A MFr.DF Bld
Synonyms
Hgb A MFr.DF Bld
Category
hematology
Sample
whole-blood
Container
Lavender-top (EDTA)
Units
N/A
Ref low
12
Ref high
17.5
Critical range
7 N/A – 20 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
85018
SNOMED
900000000000003004
Specimen volume
3 mL
Stability
Room temperature: 4h, Refrigerated: 24h
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What Is This Test Measuring?

Flow cytometry is a technique that analyzes the size, shape, and internal components of cells, including hemoglobin content. By measuring the mass fraction of hemoglobin in your red blood cells, this test can provide insights into their overall health and functionality. It may be used alongside other blood tests to diagnose conditions like anemia or hemolytic disorders.

Why Might You Need This Test?

Your healthcare provider may order this test if you have symptoms like fatigue, pale skin, or unusual bruising, or if they suspect conditions such as thalassemia, sickle cell disease, or other hemoglobin-related disorders. It can also be part of a broader evaluation for unexplained anemia or blood abnormalities.

How Is the Sample Collected?

A small amount of blood is drawn from a vein in your arm, typically in a clinic or lab setting. The blood sample is then processed to isolate red blood cells for analysis.

What Do the Results Mean?

Results provide a qualitative or quantitative assessment of hemoglobin distribution within red blood cells. Abnormal findings may indicate issues such as increased or decreased hemoglobin content, which can guide further diagnostic steps or treatment planning.

Reference range

Adult males: 13.5-17.5 g/dL; Females: 12.0-16.0 g/dLN/A

Specimen: whole-blood

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Hemoglobin Mass Fraction (RBC)

Frequently asked questions

How often do I need this test?
The frequency of this test depends on your symptoms, medical history, and ongoing health conditions. Your healthcare provider will determine the best schedule based on your individual needs. Regular monitoring may be recommended if you have a known hemoglobin disorder or related conditions.
What are the typical reference ranges for this test?
This test does not have a single numeric reference range because it evaluates hemoglobin mass fraction qualitatively or semi-quantitatively. Results are interpreted in the context of your clinical picture and compared to expected distributions for healthy individuals.
Can I prepare for this test in any special way?
No special preparation is typically required for this test. However, avoid strenuous activity or eating heavy meals 12 hours before your blood draw if instructed by your provider.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy