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Hemoglobin (Hb) Concentration in Whole Blood

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of hemoglobin in your blood, which carries oxygen to your body’s tissues. Hemoglobin levels help doctors assess whether you have enough red blood cells and healthy blood flow.

Quick summary

["Hemoglobin levels indicate your body’s ability to deliver oxygen effectively."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
32140-6
Short code
Hgb F Bld Ql Kleih Betke
Synonyms
Hgb F Bld Ql Kleih Betke
Category
hematology
Sample
whole-blood
Container
Lavender-top (EDTA)
Units
N/A
Ref low
12
Ref high
17.5
Critical range
7 N/A – 20 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
kleihauer-betke
CPT
85018
SNOMED
900000000000003004
Specimen volume
3 mL
Stability
Room temperature: 4h, Refrigerated: 24h
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Matters

Hemoglobin is a protein in red blood cells that binds and transports oxygen from your lungs to the rest of your body. Low levels may suggest anemia, blood loss, or other health conditions, while high levels could indicate conditions like polycythemia or dehydration. Your doctor may order this test to diagnose or monitor a variety of medical issues, including fatigue, shortness of breath, or unusual pale skin.

What to Expect Before the Test

This test is typically done by drawing blood from a vein in your arm. You may feel a slight pinch, but it usually takes only a few minutes. No special preparation is needed unless your doctor instructs otherwise. If you’re taking certain medications or have a history of bleeding disorders, let your healthcare provider know before the test.

What the Results Mean

Hemoglobin results are compared to a reference range based on your age, sex, and overall health. While reference ranges can vary slightly between labs, they generally fall within a specific range for healthy individuals. Your doctor will discuss your results in the context of your symptoms and medical history to determine if further action is needed.

Common Conditions Affecting Hemoglobin

Low hemoglobin levels (anemia) may be caused by conditions like iron deficiency, chronic illness, or blood loss. High levels could result from conditions like dehydration, living at high altitudes, or certain blood disorders. Your doctor may recommend additional tests or treatments based on the underlying cause of your hemoglobin levels.

Reference range

Adult males: 13.5-17.5 g/dL; Females: 12.0-16.0 g/dLN/A

Specimen: whole-blood

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Hb Level

Frequently asked questions

How often will I need this test?
The frequency of hemoglobin tests depends on your health status. Routine blood tests, such as a complete blood count (CBC), often include hemoglobin measurement. If you have a chronic condition like anemia or kidney disease, your doctor may order this test more regularly to monitor your progress.
What happens if my hemoglobin levels are out of range?
If your hemoglobin levels are lower than expected, your doctor may recommend additional tests to identify the cause, such as checking iron stores, vitamin levels, or blood loss. If levels are high, they may investigate potential causes like dehydration or polycythemia. Treatment plans will be tailored to your specific needs and may include lifestyle changes, medications, or further medical evaluation.
Can I eat or drink anything before this test?
For most hemoglobin tests, you can eat and drink normally before the procedure. However, if your doctor has specific instructions (such as fasting), follow them to ensure accurate results. Avoid smoking or consuming alcohol for at least 24 hours before the test if recommended.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy