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High-Performance Liquid Chromatography (HPLC) Analysis of Hemoglobin F Fraction

Published: August 9, 2026Updated: August 16, 2026

This test measures the proportion of fetal hemoglobin (HbF) in your blood using high-performance liquid chromatography. HbF is a form of hemoglobin found in newborns and some adults with certain blood disorders.

Quick summary

["Measuring HbF can help diagnose or monitor conditions like beta-thalassemia or sickle cell disease."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
42246-9
Short code
Hgb F MFr Bld HPLC
Synonyms
Hgb F MFr Bld HPLC
Category
hematology
Sample
whole-blood
Container
Lavender-top (EDTA)
Units
N/A
Ref low
12
Ref high
17.5
Critical range
7 N/A – 20 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
hplc
CPT
85018
SNOMED
900000000000003004
Specimen volume
3 mL
Stability
Room temperature: 4h, Refrigerated: 24h
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test to evaluate blood disorders, track disease progression, or assess treatment effectiveness. It’s often used in patients with inherited blood conditions or those undergoing blood transfusions.

How the Test Works

A small blood sample is collected and analyzed using HPLC to separate and measure different hemoglobin types, including HbF. This method provides precise data on HbF levels without relying on complex calculations.

Sample Preparation

No special preparation is needed, but fasting may be advised depending on your provider’s instructions. Wear a comfortable shirt for easy access to your arm.

What to Expect During the Test

The test involves a quick venipuncture (blood draw) from a vein in your arm. You may feel a slight pinch, but it usually takes only a few minutes. You can resume normal activities immediately afterward.

Reference range

Adult males: 13.5-17.5 g/dL; Females: 12.0-16.0 g/dLN/A

Specimen: whole-blood

No special preparation required. Inform provider of any chest pain or symptoms.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

HPLC HbF Analysis

Frequently asked questions

What does an abnormal HbF result mean?
An abnormal HbF level may suggest an inherited blood disorder, a medical condition affecting red blood cells, or other underlying health issues. Your provider will discuss the results with you and recommend next steps, such as further testing or monitoring.
How often should I get this test?
Frequency depends on your diagnosis and treatment plan. If you have a known blood disorder, your provider may recommend regular monitoring. If this is a one-time screening, follow-up may not be needed unless symptoms arise.
Can lifestyle changes affect HbF levels?
While lifestyle factors like diet or exercise generally don’t directly influence HbF levels, they can impact overall health. If you have a blood disorder, your provider may advise specific dietary or activity guidelines to support your treatment.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy