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Hemoglobin F Percentage by Electrophoresis (Blood)

Published: August 9, 2026Updated: August 16, 2026

This test measures the percentage of fetal hemoglobin (HbF) in your red blood cells. Fetal hemoglobin is a type of hemoglobin found in newborns and some adults with certain blood disorders.

Quick summary

["HbF levels can help diagnose or monitor conditions like thalassemia or sickle cell disease."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
13514-5
Short code
Hgb Fract Bld Elph-Imp
Synonyms
Hgb Fract Bld Elph-Imp
Category
hematology
Sample
whole-blood
Container
Lavender-top (EDTA)
Units
N/A
Ref low
12
Ref high
17.5
Critical range
7 N/A – 20 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
electrophoresis
CPT
85018
SNOMED
900000000000003004
Specimen volume
3 mL
Stability
Room temperature: 4h, Refrigerated: 24h
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you have symptoms of blood disorders, such as fatigue, pale skin, or frequent infections. It may also be used to screen for genetic blood conditions or to monitor treatment progress in people with known hemoglobin disorders.

What to Expect Before the Test

No special preparation is needed for this test. A blood sample will be taken from a vein in your arm, similar to a regular blood draw. You may feel a slight pinch, but it usually doesn’t cause discomfort.

What the Test Results Mean

Hemoglobin F is normally present in small amounts in adults. Higher levels may indicate conditions like beta-thalassemia, sickle cell disease, or other hemoglobinopathies. Lower levels in newborns can be normal or may require further evaluation.

Common Conditions Monitored

This test is often used to diagnose or manage conditions such as thalassemia, sickle cell disease, or other inherited blood disorders that affect hemoglobin production.

Reference range

Adult males: 13.5-17.5 g/dL; Females: 12.0-16.0 g/dLN/A

Specimen: whole-blood

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

HbF % (Electrophoresis)

Frequently asked questions

What is fetal hemoglobin (HbF)?
Fetal hemoglobin is a type of hemoglobin found in newborns that helps carry oxygen more efficiently in the womb. In adults, it usually makes up a small percentage of total hemoglobin, but certain blood disorders can cause it to increase.
Why might my HbF levels be checked?
Your provider may check HbF levels if you have symptoms of blood disorders, a family history of hemoglobinopathies, or if you are being treated for conditions like thalassemia or sickle cell disease.
How long will it take to get my results?
Results are typically available within a few days to a week, depending on your lab’s processing schedule. Your healthcare provider will discuss the results with you during your follow-up visit.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy