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Free Hemoglobin in Plasma (Mass Concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of free hemoglobin (the protein that carries oxygen in your blood) that is not bound to other substances in your plasma.

Quick summary

["Helps assess conditions affecting red blood cells or blood breakdown."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
721-1
Short code
Hgb Free Plas-mCnc
Synonyms
Hgb Free Plas-mCnc
Category
hematology
Sample
plasma
Container
Lavender-top (EDTA) or Green-top (Heparin)
Units
N/A
Ref low
12
Ref high
17.5
Critical range
7 N/A – 20 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
85018
SNOMED
900000000000003004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your doctor may request this test if you have symptoms like fatigue, pale skin, or unusual bruising, or if they suspect conditions such as hemolysis (red blood cell destruction), anemia, or certain blood disorders. It can also help monitor treatments for blood-related conditions.

What to Expect Before the Test

You’ll typically have a small needle inserted into your arm to draw a blood sample. This is usually quick and painless. No special preparation is needed, but inform your doctor about any medications or supplements you take.

How the Test Results Are Used

Results help doctors diagnose or monitor conditions affecting red blood cells, such as whether your body is breaking down red blood cells too quickly or if there’s an issue with how hemoglobin functions in your blood.

Common Conditions Monitored

This test is often used alongside other blood tests to check for hemolytic anemia, sickle cell disease, or conditions involving abnormal hemoglobin production.

Reference range

Adult males: 13.5-17.5 g/dL; Females: 12.0-16.0 g/dLN/A

Specimen: plasma

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Free Hemoglobin Plasma

Frequently asked questions

What does a high free hemoglobin level mean?
A high level may signal red blood cells are breaking down too quickly (hemolysis) or that you have conditions like sickle cell disease, severe dehydration, or certain medications affecting blood. Your doctor will review your symptoms and other tests to determine the cause.
What does a low free hemoglobin level mean?
A low level might suggest problems with red blood cell production, such as anemia, or conditions where hemoglobin isn’t functioning properly. It could also be related to blood loss or certain genetic disorders.
Does this test require fasting?
No fasting is required for this test. However, inform your doctor about any medications, supplements, or recent blood donations that could affect results.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy