Serum or plasma protein electrophoresis with bromocresol green and bromocresol purple staining

What Proteins Does This Test Measure?

Electrophoresis separates blood proteins based on size and charge, creating distinct bands. Bromocresol green and bromocresol purple dyes bind to specific proteins, highlighting albumin (fast-moving) and globulins (slower-moving). This helps identify shifts in protein levels that may indicate conditions like liver disease, kidney problems, or immune disorders.

Why Might You Need This Test?

Your doctor may order this test if you have symptoms like swelling, unexplained weight loss, fatigue, or signs of liver/kidney issues. It’s also used to monitor conditions like multiple myeloma, chronic inflammation, or nutritional deficiencies.

Common Uses and Conditions

This test is often used to diagnose or monitor conditions affecting protein production or balance, such as liver disease (e.g., cirrhosis), kidney disease, or certain cancers like multiple myeloma. It can also help evaluate nutritional status or detect autoimmune disorders.

How Is the Test Performed?

A blood sample is drawn, and the serum or plasma is placed in a gel or medium. An electric current is applied to separate proteins into bands. After staining with bromocresol dyes, the pattern of bands is analyzed for relative amounts of albumin and globulins.