Serum or plasma protein electrophoresis with bromocresol purple dye binding assay

What Proteins Does This Test Measure?

Electrophoresis separates blood proteins into different fractions based on their size and charge. The bromocresol purple dye binds to albumin and globulins, allowing for visual or quantitative assessment of each fraction. Key proteins include albumin (the most abundant protein, which helps maintain fluid balance) and various globulins (antibodies and other proteins involved in immune response and transport).

Why Might This Test Be Ordered?

Your healthcare provider may order this test to diagnose or monitor conditions such as liver disease (e.g., cirrhosis), kidney disease, multiple myeloma, or other protein-related disorders. It can also help assess nutritional status or detect abnormal protein production.

Sample Collection and Preparation

A blood sample is drawn from a vein, usually in your arm. The blood is then processed to separate serum (liquid part after clotting) or plasma (liquid part before clotting). The sample is sent to a lab where proteins are separated using an electric field and analyzed with the bromocresol purple dye.

Interpreting Results

Results show the relative amounts of different protein fractions. Abnormal patterns may indicate conditions like hypoalbuminemia (low albumin), hyperglobulinemia (high globulins), or specific types of protein abnormalities linked to diseases.