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Hemoglobin Mass Fraction in Whole Blood

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of hemoglobin (the protein in red blood cells that carries oxygen) present in your blood.

Quick summary

["Helps assess overall red blood cell function and oxygen-carrying capacity."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
51613-8
Short code
Hgb J MFr Bld
Synonyms
Hgb J MFr Bld
Category
hematology
Sample
whole-blood
Container
Lavender-top (EDTA)
Units
N/A
Ref low
12
Ref high
17.5
Critical range
7 N/A – 20 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
85018
SNOMED
900000000000003004
Specimen volume
3 mL
Stability
Room temperature: 4h, Refrigerated: 24h
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What Is Hemoglobin?

Hemoglobin is the key protein in red blood cells that binds and transports oxygen from your lungs to the rest of your body. It also helps remove carbon dioxide. The amount of hemoglobin in your blood can give doctors important clues about your health, especially concerning anemia or blood disorders.

Why Might You Need This Test?

Your healthcare provider may order this test if you have symptoms like fatigue, pale skin, shortness of breath, or dizziness. It can also be part of routine blood checks or when investigating conditions like anemia, blood loss, or certain blood disorders.

When Is This Test Performed?

This test is typically done using a simple blood draw from a vein in your arm. The sample is collected in a tube and sent to a lab for analysis. Results are usually available within a few days.

How Does This Test Work?

The test measures the mass fraction of hemoglobin in whole blood, which reflects the total amount of hemoglobin present. This is often done using advanced laboratory techniques like mass spectrometry or high-performance liquid chromatography (HPLC).

Reference range

Adult males: 13.5-17.5 g/dL; Females: 12.0-16.0 g/dLN/A

Specimen: whole-blood

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Hemoglobin Mass Fraction

Frequently asked questions

What Are Common Reasons for This Test?
This test is often used alongside other blood tests to diagnose anemia, evaluate chronic diseases, or assess blood health before surgeries or treatments.
How Long Does It Take to Get Results?
Results are usually available within 1 to 3 business days, depending on your lab’s processing time.
Can I Fast Before This Test?
No fasting is required for this test, as it involves a simple blood draw.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy