Serum Protein Electrophoresis (Qualitative) with Bromocresol Purple Staining
This test measures the distribution of different protein fractions in your blood serum using electrophoresis, a technique that separates proteins based on size and charge. The bromocresol purple dye helps visualize specific protein bands.
["Helps identify abnormal protein patterns that may signal conditions like multiple myeloma, liver disease, or other disorders."]
Key facts at a glance
- LOINC
- 4602-9
- Short code
- Hgb Kempsey Bld Ql Elph Alk
- Synonyms
- Hgb Kempsey Bld Ql Elph Alk
- Category
- hematology
- Sample
- whole-blood
- Container
- Lavender-top (EDTA)
- Units
- N/A
- Ref low
- 3.5
- Ref high
- 5
- Critical range
- 7 N/A – 20 N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- electrophoresis-ph
- CPT
- 84132
- SNOMED
- 110742001
- Specimen volume
- 3 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h
- Hemolysis max
- 0
- Lipemia max
- 0
- Icterus max
- 0
- TAT
- 4–24 hours
- Order
- routine
What This Test Measures
Electrophoresis separates proteins into distinct bands based on their electrical charge and molecular weight. The bromocresol purple stain highlights certain protein groups, including albumin, alpha-globulins, beta-globulins, and gamma-globulins. This helps detect shifts in protein composition that may indicate underlying health issues.
How the Test Works
A small blood sample is drawn and placed in a gel medium. An electric current is applied, causing proteins to migrate toward the positive electrode. After staining, the bands are analyzed to compare protein levels across different fractions.
Common Uses
This test is often used to diagnose conditions such as multiple myeloma (a cancer of plasma cells), liver disease, kidney disease, or inflammatory disorders. It may also help monitor treatment progress or identify hidden protein abnormalities.
Sample Collection
A simple blood draw is performed in a clinic or lab. You may be asked to fast for a few hours before the test, depending on your doctor’s instructions. Avoid strenuous activity or certain medications that could affect protein levels.
Reference range
Adult: 3.5-5.0 mmol/LN/A
No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Clinical Significance
Short name
Protein Electrophoresis (Serum)
Frequently asked questions
Why might my doctor order this test?
What do abnormal results mean?
How long does it take to get results?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy