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Hemoglobin Mass by Flow Cytometry (Blood)

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of hemoglobin in your red blood cells, which helps assess iron stores and overall red blood cell health.

Quick summary

["Helps diagnose conditions affecting red blood cells or iron levels."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
53224-2
Short code
Hgb M Bld Ql
Synonyms
Hgb M Bld Ql
Category
hematology
Sample
whole-blood
Container
Lavender-top (EDTA)
Units
N/A
Ref low
12
Ref high
17.5
Critical range
7 N/A – 20 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
85018
SNOMED
900000000000003004
Specimen volume
3 mL
Stability
Room temperature: 4h, Refrigerated: 24h
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Matters

Hemoglobin is the protein in red blood cells that carries oxygen throughout your body. Low levels can indicate anemia or other blood disorders. This test provides a precise measurement of hemoglobin mass, which can help doctors identify underlying issues like iron deficiency or other nutrient deficiencies.

What to Expect Before the Test

No special preparation is needed. You’ll have a small needle inserted into your arm or hand to draw a small blood sample. The process is quick and typically painless.

How the Test Works

Blood is analyzed using flow cytometry, a technique that separates and counts red blood cells while measuring their hemoglobin content. This method is highly accurate and non-invasive.

Possible Results and Next Steps

Results may be compared to standard ranges for your age and sex. If your levels are low, your doctor may recommend further tests, such as a complete blood count (CBC) or iron studies, to determine the cause.

Reference range

Adult males: 13.5-17.5 g/dL; Females: 12.0-16.0 g/dLN/A

Specimen: whole-blood

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Hemoglobin Mass Quantification

Frequently asked questions

What does a low or high hemoglobin mass mean?
Low hemoglobin mass can signal anemia, which may be caused by factors like poor diet, chronic illness, or blood loss. High levels may be seen in conditions like polycythemia (overproduction of red blood cells). Your doctor will discuss findings in the context of your overall health.
How often should I get this test?
This test is often part of a broader blood evaluation, such as a CBC, and may be recommended periodically if you have conditions like anemia, kidney disease, or other chronic health concerns. Follow your doctor’s advice for timing.
Can diet or lifestyle affect this test?
Yes, diet rich in iron, vitamin B12, and folate can influence red blood cell production. Avoiding alcohol and managing chronic conditions like diabetes or kidney disease may also impact results. Discuss dietary habits with your healthcare provider.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy