Hemoglobin M (Methaemoglobin) Screening or Confirmation by High-Performance Liquid Chromatography (HPLC)
This test measures the presence of abnormal hemoglobin variants, specifically methaemoglobin, which can affect how oxygen is carried in your blood. Methaemoglobin is a form of hemoglobin that cannot effectively transport oxygen, potentially leading to oxygen deprivation in tissues.
["This test helps identify genetic conditions that may cause oxygen-related symptoms like fatigue or shortness of breath."]
Key facts at a glance
- LOINC
- 71860-1
- Short code
- Hgb M MFr.DF Bld
- Synonyms
- Hgb M MFr.DF Bld
- Category
- hematology
- Sample
- whole-blood
- Container
- Lavender-top (EDTA)
- Units
- N/A
- Ref low
- 12
- Ref high
- 17.5
- Critical range
- 7 N/A – 20 N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- CPT
- 85018
- SNOMED
- 900000000000003004
- Specimen volume
- 3 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h
- Hemolysis max
- 0
- Lipemia max
- 0
- Icterus max
- 0
- TAT
- 4–24 hours
- Order
- routine
Why This Test Might Be Ordered
Your healthcare provider may recommend this test if you have symptoms like pale skin, fatigue, shortness of breath, or cyanosis (blue-tinged skin). It may also be part of a genetic screening or evaluation for conditions like methemoglobinemia, a rare blood disorder.
What to Expect Before the Test
No special preparation is needed. You may donate whole blood, and the test is typically performed on a small sample. If you’re using blood thinners, let your provider know to avoid unnecessary delays.
How the Test Works
The test uses high-performance liquid chromatography (HPLC) to separate and identify different types of hemoglobin in your blood. This method helps detect abnormal hemoglobin variants, including methaemoglobin, which may not be visible with standard blood tests.
What the Results Mean
Results may indicate an abnormal level of methaemoglobin or other hemoglobin variants. Elevated levels could signal a genetic condition requiring further evaluation by a specialist.
Reference range
Adult males: 13.5-17.5 g/dL; Females: 12.0-16.0 g/dLN/A
No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Detecting methaemoglobin early is crucial because it can lead to chronic oxygen deprivation. If confirmed, this condition may require lifestyle adjustments, dietary changes, or medical treatments to manage symptoms and prevent complications.
Short name
Hemoglobin M (HPLC)
Frequently asked questions
What causes abnormal hemoglobin levels in this test?
Will I need follow-up tests if my results are abnormal?
Can I prevent this condition from developing?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy