Hydroxyproline in Urine (24-Hour Collection)

Why This Test Is Done

Your doctor may order this test to evaluate conditions that affect collagen production or breakdown, such as certain types of kidney disease, scarring (fibrosis), or connective tissue disorders. It can also be used to monitor treatment responses in patients with chronic kidney disease or other collagen-related conditions.

What to Expect Before the Test

You’ll need to collect all urine for a full 24-hour period, starting right after you wake up and ending just before you wake up the next morning. Drink plenty of fluids to help your urine flow normally. Avoid taking any medications that might interfere with the test unless approved by your doctor.

How the Test Is Done

Your urine sample is collected in a container and sent to a lab. The lab analyzes the sample for hydroxyproline levels using specialized techniques, such as chromatography or immunoassay, to measure its concentration.

What the Results Mean

Results are typically reported as the total amount of hydroxyproline excreted in the urine over 24 hours. Higher levels may indicate increased collagen breakdown, while lower levels could suggest reduced collagen turnover or certain metabolic conditions.