Serum Protein Electrophoresis (Qualitative) with Bromocresol Purple Staining
This test measures the distribution of different protein types in your blood serum using electrophoresis, a technique that separates proteins based on size and charge. The bromocresol purple dye helps visualize protein bands, which can indicate abnormalities in protein levels.
["This test helps identify potential protein disorders by showing how proteins are distributed in your blood."]
Key facts at a glance
- LOINC
- 4629-2
- Short code
- Hgb Zurich Bld Ql Elph Acid
- Synonyms
- Hgb Zurich Bld Ql Elph Acid
- Category
- hematology
- Sample
- whole-blood
- Container
- Lavender-top (EDTA)
- Units
- N/A
- Ref low
- 12
- Ref high
- 17.5
- Critical range
- 7 N/A – 20 N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- electrophoresis-ph
- CPT
- 85018
- SNOMED
- 900000000000003004
- Specimen volume
- 3 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h
- Hemolysis max
- 0
- Lipemia max
- 0
- Icterus max
- 0
- TAT
- 4–24 hours
- Order
- routine
What Does This Test Measure?
Electrophoresis separates proteins in your blood into distinct bands based on their electrical charge and size. Each band corresponds to a group of proteins, such as albumin, globulins (including immunoglobulins), and other fractions. The bromocresol purple dye stains these proteins, allowing for visual assessment of their relative amounts.
Why Might You Need This Test?
This test is often used to diagnose conditions affecting protein levels, such as multiple myeloma (a cancer of plasma cells), systemic lupus erythematosus, or other autoimmune diseases. It may also help monitor treatment progress or detect subtle protein abnormalities in chronic diseases.
Common Uses and Indications
Common reasons for this test include evaluating symptoms like unexplained weight loss, fatigue, or swollen lymph nodes. It may also be part of a broader workup for kidney disease, liver disease, or inherited protein disorders.
Sample Collection and Preparation
A blood sample is drawn from a vein, typically in your arm. The serum is separated from blood cells and prepared for electrophoresis. The bromocresol purple dye is added to stain the proteins, which are then analyzed under a microscope.
Reference range
Adult males: 13.5-17.5 g/dL; Females: 12.0-16.0 g/dLN/A
No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Abnormal protein patterns on electrophoresis can reveal underlying conditions like monoclonal gammopathy (excessive production of a single type of antibody), hypoalbuminemia (low albumin levels), or increased globulin levels. These findings may guide further testing or treatment decisions.
Short name
Serum Protein Electrophoresis (Qualitative)
Frequently asked questions
What does a 'qualitative' result mean?
How long does it take to get results?
Can this test be done at home?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy