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HIV Antibody Screening and Confirmatory Test (Serum/Plasma)

Published: August 9, 2026Updated: August 16, 2026

This test detects antibodies to the human immunodeficiency virus (HIV), which your body makes in response to the virus. It helps determine if you’ve been exposed to HIV.

Quick summary

["This test screens for HIV antibodies, which can indicate past or current infection."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
80203-3
Short code
HIV 1 & 2 Ab SerPlBld IA.rapid
Synonyms
HIV 1 & 2 Ab SerPlBld IA.rapid
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ia-rapid
CPT
86701
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

How the Test Works

The test uses a blood sample (either serum or plasma) to identify HIV antibodies through an immunoassay method. This detects proteins your immune system produces after exposure to HIV, helping confirm infection.

What to Expect Before the Test

No special preparation is needed. Bring a photo ID if required by your healthcare provider. Avoid alcohol for 24 hours before testing if instructed.

What to Expect During the Test

A healthcare professional will draw blood from a vein in your arm. You may feel a slight pinch, but the procedure is quick and usually painless. The blood sample is then processed to extract serum or plasma for testing.

What to Expect After the Test

You can return to your normal activities immediately. Minor bruising or soreness at the injection site may occur but should resolve within a few days. Follow-up instructions from your provider may include waiting for results or scheduling a confirmatory test if needed.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Positive results indicate the presence of HIV antibodies, suggesting past or current infection. A positive screening test may require a confirmatory test to ensure accuracy. Early detection is crucial for managing HIV and preventing complications. Negative results mean no detectable antibodies at the time of testing, but exposure cannot be ruled out without retesting.

Short name

HIV Antibody Test

Frequently asked questions

Why was I asked to take this test?
Your healthcare provider may recommend this test if you have risk factors for HIV, such as unprotected sexual contact, shared needles, or other behaviors that increase exposure risk. It’s also part of routine testing for certain groups or during medical evaluations.
What does a positive result mean?
A positive result means your blood contains antibodies to HIV, which may indicate past or current infection. You’ll need further testing to confirm the diagnosis and discuss next steps with your healthcare provider.
Can I get HIV from a blood test?
No, you cannot contract HIV from a blood test. The test only analyzes your blood for antibodies; it does not expose you to the virus.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy