Skip to content

Serum Protein Electrophoresis with Bromocresol Green (BCG) Dye Binding Assay

Published: August 9, 2026Updated: August 16, 2026

This test measures the distribution of proteins in your blood, including albumin and globulins, using a dye-binding method to assess protein quality and potential abnormalities.

Quick summary

["This test helps identify protein disorders or nutritional deficiencies by analyzing blood protein patterns."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
94442-1
Short code
HLA class I Ab.IgG prozone SerPl FC
Synonyms
HLA class I Ab.IgG prozone SerPl FC
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
flow-cytometry
CPT
82784
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

The test uses a dye (bromocresol green) that binds differently to various proteins in your serum. Albumin, globulins, and other proteins separate based on size and charge, allowing for a visual and quantitative assessment of protein fractions.

Why It’s Done

Doctors may order this test to diagnose conditions like hypoalbuminemia (low albumin), protein-losing disorders, or to monitor chronic diseases affecting protein synthesis or breakdown.

Sample Collection and Preparation

Blood is drawn from a vein, typically in your arm. The serum (liquid part after clotting) is separated and tested for protein distribution using electrophoresis and dye binding.

Common Conditions Monitored

This test helps detect abnormalities in protein levels that may indicate kidney disease, liver dysfunction, malnutrition, or autoimmune conditions affecting protein production.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: Protein Electrophoresis with BCG Dye Binding is critical for identifying imbalances in protein fractions. Low albumin may signal malnutrition or kidney issues, while abnormal globulin patterns could suggest immune disorders or chronic inflammation. Early detection can guide treatment decisions.

Short name

BCG Protein Electrophoresis

Frequently asked questions

How long does it take to get results from this test?
Results are typically available within 1–3 business days, depending on lab processing times. Your healthcare provider will discuss findings with you during your follow-up visit.
What does a normal result mean?
A normal result shows balanced protein fractions, with albumin being the most abundant and globulins distributed appropriately. However, reference ranges can vary by lab, so your provider will interpret results in the context of your health.
Can diet affect this test?
Yes, diet may influence protein levels. High-protein diets or certain medical conditions (e.g., kidney disease) could alter results. Your provider may recommend dietary adjustments or retesting if needed.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy