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Anti-D (RhD) Antibody Screening and Confirmation (Serum/Plasma)

Published: August 9, 2026Updated: August 16, 2026

This test checks for the presence of antibodies against the RhD antigen in your blood, which can affect blood compatibility during transfusions or pregnancy.

Quick summary

["Your doctor may order this test if you’ve had a blood transfusion, pregnancy complications, or if you’re Rh-negative and planning pregnancy."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
94439-7
Short code
HLA-DRB1 IgG SerPl FC
Synonyms
HLA-DRB1 IgG SerPl FC
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
flow-cytometry
CPT
82784
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Matters for Blood Safety

The RhD antigen is a protein on red blood cells. If your immune system mistakenly identifies it as foreign, it may produce antibodies (like anti-D) that can cause problems during blood transfusions or pregnancy. This test screens for these antibodies first, then confirms their presence if needed.

How the Test Works

The test uses a method called **immunoassay** to detect anti-D antibodies in your serum or plasma. If antibodies are found, a confirmatory test ensures accuracy before proceeding with further evaluation.

When You Might Need This Test

You may need this test if you’ve had a blood transfusion, miscarriage, stillbirth, or if you’re Rh-negative and pregnant. Doctors also order it for people with autoimmune conditions or those at risk for hemolytic disease of the fetus/newborn (HDFN).

What to Expect Before the Test

No special preparation is needed. You’ll provide a blood sample (usually from your arm) in a lab. The test checks for antibodies that could interfere with safe blood transfusions or pregnancy outcomes.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Positive results indicate the presence of anti-D antibodies, which can lead to complications like hemolytic anemia or HDFN if untreated. Early detection allows for interventions like Rh immune globulin (RhIG) to prevent harm during pregnancy or transfusions.

Short name

RhD Antibody Test

Frequently asked questions

What does a positive result mean?
A positive result means your blood contains anti-D antibodies, which may require further testing or treatment (like RhIG) to prevent complications during pregnancy or blood transfusions.
Can I get this test at home?
No, this test is done in a clinical lab using a blood sample. Your healthcare provider will schedule it during a doctor’s visit or lab visit.
How long does it take to get results?
Results are typically available within 1–2 business days, depending on lab processing times.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy