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Serum or Plasma Substance Concentration (Bromocresol Green Dye Binding)

Published: August 9, 2026Updated: August 16, 2026

This test measures how bromocresol green dye binds to a protein in your blood, often used to assess liver function or protein synthesis.

Quick summary

["This test helps evaluate liver health or protein-related conditions by tracking dye-protein interactions."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
72910-3
Short code
HNA 4a/4b Ab Ser Ql IA
Synonyms
HNA 4a/4b Ab Ser Ql IA
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ia
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

The test involves mixing a sample of your blood with bromocresol green dye. The dye binds to certain proteins, and the amount bound is measured to reflect overall protein levels or liver function. This method can indirectly assess conditions affecting protein synthesis or liver metabolism.

Why It’s Done

Your healthcare provider may order this test if you have symptoms like jaundice, unexplained weight loss, or signs of liver disease. It can also help monitor conditions like cirrhosis or protein deficiencies.

Common Conditions Monitored

This test is less commonly used today but historically helped diagnose liver disease, protein disorders, or metabolic issues by tracking dye-protein interactions.

What to Expect Before the Test

No special preparation is needed, but fasting may be required depending on your specific order. Bring any medications or questions to your appointment.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

BCG Dye Binding Test

Frequently asked questions

How long does it take for results to be available?
Results typically arrive within 24 to 48 hours, depending on lab processing times. Follow up with your provider for interpretation.
Can I drink water before this test?
Check with your provider first—some tests require fasting, while others do not. Avoid caffeine or alcohol if instructed.
Does this test require fasting?
Your provider will specify if fasting is needed. If unsure, ask during your appointment.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy