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Horseradish Peroxidase (HRP) Activity in Serum/Plasma

Published: August 9, 2026Updated: August 16, 2026

This test measures the activity of horseradish peroxidase, an enzyme naturally present in certain plants and used in some medical tests to detect or quantify other substances.

Quick summary

["HRP activity may be involved in certain diagnostic or research processes, often linked to enzyme-linked immunoassays (ELISA)."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
7418-7
Short code
Horse Dander BasoBnd Ab Qn
Synonyms
Horse Dander BasoBnd Ab Qn
Category
chemistry
Sample
whole-blood
Container
Lavender-top (EDTA)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
lhr
Specimen volume
3 mL
Stability
Room temperature: 4h, Refrigerated: 24h
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What Is Horseradish Peroxidase Used For?

Horseradish peroxidase (HRP) is primarily used in laboratory settings as an enzyme marker in immunoassays. It helps amplify the detection of specific antibodies or antigens by catalyzing a chemical reaction that produces a measurable signal. This can be part of screening or confirmatory tests for conditions like autoimmune diseases, infections, or certain cancers.

How Is This Test Performed?

A blood sample (serum or plasma) is collected and analyzed using an immunoassay method, which may involve exposing the sample to HRP-labeled antibodies. The enzyme’s activity is then measured to determine its concentration or functional role in the test.

Who Might Need This Test?

This test is not commonly ordered for routine patient care but may be used in specialized research, diagnostic research protocols, or specific clinical studies where HRP-based assays are employed. Your healthcare provider may recommend it if you’re part of a study or have a condition requiring enzyme-linked detection.

What Does a Result Mean?

Interpretation depends entirely on the context of the test, such as whether it’s part of a screening, research study, or confirmatory assay. Results may indicate enzyme activity levels relevant to the specific assay being performed, but general reference ranges are not applicable here.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

HRP Activity

Frequently asked questions

Why would my doctor order this test?
This test is unlikely to be ordered for routine medical reasons. It may be part of a research study, specialized diagnostic protocol, or quality control process in a laboratory setting. If you’re unsure, discuss your test results with your healthcare provider.
What does a high or low HRP activity result mean?
Since HRP activity is used as an enzyme marker in assays—not as a standalone clinical test—results are interpreted within the context of the specific test being performed. A deviation from expected assay performance (rather than a numeric threshold) may indicate issues with the sample, reagents, or test protocol.
Can this test be done at home?
No, this test requires a laboratory setting and is not available for home use. Blood samples must be collected by a healthcare professional and processed in a clinical or research lab.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy