Skip to content

Hormone Profile: Adrenocorticotropic Hormone (ACTH) and Cortisol (24-hour urine)

Published: August 9, 2026Updated: August 16, 2026

This test measures your body’s production of ACTH and cortisol over a 24-hour period to help diagnose conditions related to your adrenal glands or pituitary gland.

Quick summary

["Assesses adrenal or pituitary function by tracking hormone levels over time."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
50600-6
Short code
HPA-3 Bld
Synonyms
HPA-3 Bld
Category
chemistry
Sample
whole-blood
Container
Lavender-top (EDTA)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
3 mL
Stability
Room temperature: 4h, Refrigerated: 24h
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if you have symptoms like unexplained weight changes, fatigue, high blood pressure, or signs of hormonal imbalances. It’s also used to evaluate conditions like Cushing’s syndrome, adrenal insufficiency, or pituitary tumors.

What to Expect Before the Test

You’ll need a 24-hour urine collection, starting after your first morning urine. Collect all urine for the entire day, including midnight, and store it in a refrigerator. Bring the sample to the lab on the following day.

How the Test Works

Urine is tested for cortisol levels, while ACTH is often measured separately via blood or serum. The results help determine if your adrenal glands are overproducing or underproducing hormones.

Common Conditions Evaluated

This test helps diagnose or monitor conditions like Cushing’s disease (excess cortisol), Addison’s disease (adrenal insufficiency), or pituitary adenomas affecting hormone regulation.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

ACTH/Cortisol Profile

Frequently asked questions

What causes abnormal ACTH or cortisol levels?
Abnormal results may be due to hormonal imbalances, tumors (pituitary or adrenal), infections, or medications affecting hormone production. Symptoms like weight gain, muscle weakness, or fatigue can also trigger testing.
How long does it take to get results?
Results typically take 3–7 business days, depending on lab processing. Your doctor will contact you once available.
Can I take medications before the test?
Some medications (like steroids) may affect results. Always follow your doctor’s instructions—avoid changes without approval.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy