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Herpes Simplex Virus Type 2 DNA Detection by Fluorescent In Situ Hybridization (FISH) on Nucleic Acid Probe

Published: August 9, 2026Updated: August 16, 2026

This test checks for the presence of genetic material from herpes simplex virus type 2 (HSV-2) in your body fluids, often used to diagnose genital herpes.

Quick summary

["Helps confirm if you have genital herpes caused by HSV-2."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
49989-7
Short code
HSV2 DNA # Fld NAA+probe
Synonyms
HSV2 DNA # Fld NAA+probe
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
probe-amp-tar
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you have symptoms like sores, itching, or unusual discharge in the genital area, or if you have recurrent outbreaks. It can also be used to diagnose herpes in newborns or during pregnancy to assess risk to the baby.

How the Test Works

A small sample of fluid (like saliva or a swab from affected areas) is collected and analyzed using a fluorescent probe that binds to HSV-2 DNA. If the virus is present, the probe lights up under a microscope, indicating a positive result.

Sample Collection

You may need to provide a sample of saliva or a swab from your genital area during an outbreak or at other times when symptoms are present. If testing during pregnancy, your provider may collect fluid from the cervix or vagina.

Test Results Interpretation

A positive result means HSV-2 DNA is detected, strongly suggesting genital herpes. A negative result means the virus was not found in the sample at the time of testing. Results can vary depending on when testing occurs relative to symptoms.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

HSV-2 DNA Probe Test

Frequently asked questions

What does a positive result mean?
A positive result confirms the presence of HSV-2 DNA, which is the genetic material of genital herpes. This diagnosis is crucial for managing the infection, providing treatment, and preventing spread to others, especially during outbreaks or when pregnant.
How can I reduce my risk of spreading HSV-2?
To lower your risk of spreading HSV-2, avoid sexual contact during outbreaks, use barrier methods like condoms, and practice good hygiene. If you have symptoms, inform your partner(s) so they can take precautions.
Can this test be used to detect HSV-2 in asymptomatic individuals?
Yes, this test can be used in asymptomatic individuals, though results may be less reliable if testing is done outside of an active outbreak. Regular testing may be recommended for those at higher risk, such as those with recurrent symptoms or partners with known HSV-2 infection.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy