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Hemoglobin A1c (HbA1c) with 24-hour urine probe measurement

Published: August 9, 2026Updated: August 16, 2026

This test measures your average blood sugar levels over the past 2–3 months using a special urine sample collected over 24 hours. It helps assess long-term glucose control, often used in diabetes management.

Quick summary

["This test provides insight into how well your blood sugar has been managed over time, not just recent spikes or drops."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
44541-1
Short code
HTLV II DNA Bld Ql Probe
Synonyms
HTLV II DNA Bld Ql Probe
Category
chemistry
Sample
whole-blood
Container
Lavender-top (EDTA)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
probe
Specimen volume
3 mL
Stability
Room temperature: 4h, Refrigerated: 24h
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What This Test Measures

HbA1c is a form of hemoglobin (the protein in red blood cells that carries oxygen) that has attached a sugar molecule (glucose). The percentage of HbA1c in your blood reflects your average blood sugar levels over the past 2–3 months. The urine probe component involves collecting a 24-hour urine sample to indirectly assess glucose excretion, which can complement HbA1c results.

Why It’s Done

Doctors may order this test if you have diabetes or are at risk for it to monitor long-term blood sugar control. It helps track how well treatments (like medication or diet) are working over time, rather than just checking blood sugar levels at a single moment.

How It’s Performed

You’ll collect a 24-hour urine sample in a special container, starting and ending at the same time each day. The urine is tested for glucose-related substances to help evaluate how much glucose your body has processed and excreted.

What to Expect Before the Test

No special preparation is needed, but drink plenty of water to help your kidneys filter urine properly. Avoid excessive sugar intake in the 24-hour period if instructed, as it may affect results.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

HbA1c Urine Probe

Frequently asked questions

How does the urine probe differ from a standard HbA1c test?
The urine probe measures glucose excretion in your urine over 24 hours, which can indicate how much glucose your kidneys are filtering. Standard HbA1c tests measure the percentage of hemoglobin bound to glucose in your blood, giving a longer-term average. Both are useful but serve different purposes in diabetes management.
Can I eat or drink anything before giving my urine sample?
Yes, you can eat and drink normally, but avoid excessive sugar intake if your healthcare provider specifies it. Drink plenty of fluids to ensure clear urine for testing.
Why is a 24-hour urine sample needed instead of a single blood test?
A 24-hour urine sample provides a broader picture of glucose metabolism by capturing consistent excretion levels over time. Blood tests like HbA1c give a snapshot, while urine samples reflect continuous processing, helping doctors detect patterns in glucose handling.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy