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Total Bilirubin with Serum Specimen Type

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of bilirubin in your blood, a yellow pigment produced when red blood cells break down. It helps assess liver function and bile flow.

Quick summary

["Bilirubin levels can signal liver or bile duct issues."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
111327-3
Short code
HTLV I+II Ab Msmt Ser
Synonyms
HTLV I+II Ab Msmt Ser
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Matters

Bilirubin is a byproduct of hemoglobin breakdown, which occurs naturally in your body. When your liver struggles to process it, high levels can cause jaundice (yellowing of skin/eyes) or other symptoms. This test helps doctors diagnose conditions like liver disease, gallbladder problems, or hemolytic anemia.

How the Test Works

Your blood sample is drawn and sent to a lab where bilirubin is measured using a chemical reaction that detects its presence. The result is expressed in a standardized unit (though exact reference ranges vary by lab).

Common Conditions Monitored

Elevated bilirubin often signals liver or bile duct blockages (e.g., gallstones, cirrhosis), excessive red blood cell destruction (hemolysis), or liver inflammation (hepatitis). Low levels may indicate liver disease or certain genetic disorders.

What to Expect Before the Test

No special preparation is needed, but avoid alcohol or certain medications (like painkillers) for 24 hours before testing, as they may affect results.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Total Bilirubin

Frequently asked questions

What does a high bilirubin level mean?
A high bilirubin level may indicate liver dysfunction, bile duct obstruction, or red blood cell breakdown. Your doctor will consider symptoms (e.g., jaundice, fatigue) and other test results to diagnose the cause.
What does a low bilirubin level mean?
Low bilirubin can sometimes suggest liver disease (e.g., cirrhosis) or certain genetic conditions. However, normal levels alone don’t rule out underlying issues, so doctors may order additional tests.
How often should I get this test?
Your doctor will schedule this test based on your symptoms, medical history, or routine checkups. If you have liver-related conditions, they may recommend periodic monitoring.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy