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Anti-Nuclear Antibody (ANA) Profile with Immunofluorescence Probe

Published: August 9, 2026Updated: August 16, 2026

This test measures antibodies that may indicate an autoimmune condition by detecting antibodies against nuclear components in your blood. It uses a special probe to identify specific patterns of reactivity.

Quick summary

["ANA tests help diagnose conditions like lupus or other autoimmune diseases by checking for unusual immune activity."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
30159-8
Short code
HTLV I+II DNA Bld Ql NAA+probe
Synonyms
HTLV I+II DNA Bld Ql NAA+probe
Category
chemistry
Sample
whole-blood
Container
Lavender-top (EDTA)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
probe-amp-tar
Specimen volume
3 mL
Stability
Room temperature: 4h, Refrigerated: 24h
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order an ANA test if you have symptoms like joint pain, fatigue, rash, or unexplained organ issues. It’s often the first step in evaluating possible autoimmune disorders.

How the Test Works

A small blood sample is taken and exposed to a special probe that binds to nuclear material in cells. If your blood contains ANAs, they’ll react with the probe, creating visible patterns under a microscope.

What to Expect Before the Test

No special preparation is needed. You may have a slight bruise or soreness at the injection site, but this usually fades quickly.

Possible Results and Next Steps

Results are interpreted based on pattern and intensity. A positive result doesn’t always mean a diagnosis, but it may prompt further testing (like a confirmatory ANA or specific antibody tests).

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

ANA is a broad marker for autoimmune diseases. While many healthy people have low levels, elevated or specific patterns can signal conditions like systemic lupus erythematosus (SLE), rheumatoid arthritis, or mixed connective tissue disease. Always discuss results with your healthcare provider.

Short name

ANA Screen/Confirm

Frequently asked questions

What does a positive ANA test mean?
A positive ANA test means your blood contains antibodies that target nuclear components, which can occur in autoimmune diseases or sometimes in healthy individuals. It doesn’t confirm a diagnosis but may guide further testing.
Can I have false positives or negatives?
Yes, both can happen. False positives may occur with certain infections, medications, or pregnancy. False negatives are less common but possible, especially in early or mild autoimmune conditions.
How often should I get retested?
Retesting depends on your symptoms and doctor’s recommendations. If your first result was positive, follow-up may include additional tests (like anti-dsDNA or anti-Smith antibodies) or monitoring over time.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy