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Immunophenotyping of White Blood Cells (Flow Cytometry)

Published: August 9, 2026Updated: August 16, 2026

This test analyzes the types and proportions of white blood cells in your blood to identify abnormalities in cell counts or markers that may indicate conditions like infections, immune disorders, or cancers.

Quick summary

["This helps doctors diagnose and monitor blood-related diseases by checking cell types and immune function."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
34924-1
Short code
Imm Moncytes # Bld
Synonyms
Imm Moncytes # Bld
Category
chemistry
Sample
whole-blood
Container
Lavender-top (EDTA)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
3 mL
Stability
Room temperature: 4h, Refrigerated: 24h
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What This Test Measures

The test uses a specialized machine (flow cytometer) to examine specific proteins (markers) on the surfaces of different white blood cell types. It identifies and quantifies cells such as lymphocytes, monocytes, neutrophils, eosinophils, and basophils, often looking for markers like CD3, CD4, CD8, CD19, etc., which can signal immune activity or abnormalities.

Why It’s Done

Doctors may order this test to investigate symptoms like frequent infections, unexplained fevers, or to diagnose conditions such as leukemia, lymphoma, or immune deficiencies. It can also help monitor treatment progress in blood cancers or immune disorders.

Sample Collection and Preparation

A small blood sample is drawn from a vein, typically in your arm. The sample is processed and analyzed immediately or within hours to identify cell types and markers without relying on specific numeric ranges.

Common Conditions Monitored

This test is critical for diagnosing and managing blood cancers (e.g., leukemia, lymphoma), immune disorders (e.g., HIV, autoimmune diseases), and infections where white blood cell counts or functions are abnormal.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Results help determine the type and stage of blood disorders, guide treatment decisions (e.g., chemotherapy, immunotherapy), and track response to therapy. Abnormal cell counts or marker patterns may indicate underlying diseases that require further medical evaluation.

Short name

WBC Immunophenotyping

Frequently asked questions

What does an abnormal result mean?
An abnormal result may suggest an infection, immune disorder, or blood cancer. Your doctor will discuss findings in detail and may recommend additional tests or treatments based on the results.
How often is this test repeated?
Frequency depends on your condition. Some patients need this test monthly or more often during treatment, while others may only need it once or twice a year for monitoring.
Does this test involve radiation or pain?
No radiation is used. The blood draw is quick and may cause mild discomfort, but the rest of the process is painless and involves no radiation exposure.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy