Skip to content

Insulin concentration in serum/plasma (24-hour profile)

Published: August 9, 2026Updated: August 16, 2026

This test measures insulin levels over a 24-hour period using blood drawn from your arm. It helps assess how your body produces and responds to insulin, often used in diagnosing or managing conditions like diabetes.

Quick summary

["Understanding your insulin production and response over time can help manage blood sugar levels effectively."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
13608-5
Short code
Insulin 1.5h p 75 g Glc PO SerPl-mCnc
Synonyms
Insulin 1.5h p 75 g Glc PO SerPl-mCnc
Category
glucose
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2.6
Ref high
24.9
Fasting
true
Population
adult
Sex
all
Age range
18–65 years
CPT
83525
SNOMED
413618004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you have symptoms of diabetes, such as frequent urination, excessive thirst, or unexplained weight changes. It may also be used to evaluate insulin resistance, hypoglycemia, or to monitor treatment effectiveness in people with diabetes or other metabolic disorders.

How the Test Works

Blood is collected from a vein in your arm at multiple times over 24 hours. The insulin levels in your blood are measured using an immunoassay method, which detects the presence and amount of insulin in your serum or plasma.

Sample Collection Process

You’ll need to fast for a certain period before the test, usually overnight, to ensure accurate results. Blood samples are taken at scheduled intervals, and you may be asked to keep a log of your food and activity during the test period.

What to Expect Before the Test

Plan ahead by arranging for someone to drive you home if you experience drowsiness or lightheadedness from fasting. Avoid smoking, caffeine, or alcohol before the test. Wear comfortable clothing and shoes that allow easy access to your arm.

Reference range

Fasting: 2.6-24.9 μIU/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Frequently asked questions

What does a low insulin level mean?
Low insulin levels may indicate conditions such as diabetes (if insulin is insufficient to regulate blood sugar) or other metabolic issues. It can also be seen in people with certain genetic disorders or during severe stress or illness. Your doctor will interpret the results in the context of your overall health and symptoms.
What does a high insulin level mean?
High insulin levels can suggest insulin resistance, where your body doesn’t respond well to insulin, or conditions like Cushing’s syndrome or certain tumors that produce excess insulin. It may also be seen in people with obesity or certain medications.
How often should I get this test?
The frequency of this test depends on your medical condition and treatment plan. Typically, people with diabetes may have this test periodically to monitor their insulin production and adjust their treatment. Your healthcare provider will determine the best schedule for you.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy