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Serum Insulin with 24-Hour Collection (Quantitative)

Published: August 9, 2026Updated: August 16, 2026

This test measures your insulin levels over a full day to help assess how your body regulates blood sugar. It’s often used to evaluate conditions like diabetes or insulin sensitivity.

Quick summary

["This test helps doctors understand how your body responds to insulin throughout the day."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
40290-9
Short code
Insulin 1.5h p chal SerPl-sCnc
Synonyms
Insulin 1.5h p chal SerPl-sCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2.6
Ref high
24.9
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83525
SNOMED
413618004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

A 24-hour insulin test is typically ordered if you have symptoms of diabetes, insulin resistance, or unexplained blood sugar fluctuations. It may also be used to monitor treatment effectiveness in people with known metabolic disorders.

What to Expect Before the Test

You’ll need to collect blood samples every few hours for 24 hours. Your healthcare provider will guide you on proper timing, diet restrictions (like avoiding certain foods), and how to safely draw blood at home or in a clinic.

How the Test Works

Blood samples are taken from a vein (usually in your arm) at scheduled intervals. Each sample is analyzed to measure insulin concentration. The results are compared to your body’s natural insulin production patterns over time.

What the Results Mean

Results show how your insulin levels vary throughout the day. Abnormally high or low levels may indicate diabetes, hormonal imbalances, or other metabolic issues. Your doctor will interpret these results in the context of your overall health.

Reference range

Fasting: 2.6-24.9 μIU/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: Insulin Levels Over Time

Short name

24-Hour Insulin Profile

Frequently asked questions

How often do I need to fast before this test?
No fasting is required for this test. However, you should follow your doctor’s instructions regarding diet (e.g., avoiding high-sugar foods during collection times) and hydration to ensure accurate results.
Can I take my usual medications on the day of the test?
Yes, but check with your doctor first. Some medications (like insulin or blood pressure drugs) may affect insulin levels. Your provider will give specific guidance tailored to your treatment plan.
What if I miss a blood draw during the 24-hour period?
If you miss a scheduled draw, contact your healthcare provider immediately. Missing samples can skew results, so follow-up may be needed to correct the test.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy