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Insulin, Serum Concentration (24-Hour Profile)

Published: August 9, 2026Updated: August 16, 2026

This test measures insulin levels in your blood over a 24-hour period to help assess how your body responds to glucose.

Quick summary

["Understanding your insulin levels over time can help diagnose or manage conditions like diabetes or insulin resistance."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
55434-5
Short code
Insulin 20M p chal SerPl-sCnc
Synonyms
Insulin 20M p chal SerPl-sCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2.6
Ref high
24.9
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83525
SNOMED
413618004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you have symptoms of diabetes, such as frequent urination, excessive thirst, or unexplained weight changes. It may also be used to evaluate how well your body produces insulin, especially in people with suspected insulin resistance or metabolic disorders.

What to Expect Before the Test

No special preparation is typically needed. You may be asked to fast for 8–12 hours before the test, but your provider will confirm this. Bring any medications you’re currently taking, as they may affect results.

How the Test Works

Blood is collected from a vein in your arm at multiple time points over 24 hours. The samples are analyzed to measure insulin concentration at each interval, often using an immunoassay method to detect the hormone accurately.

What the Results Mean

Interpreting results involves comparing insulin levels at different times to your body’s natural glucose fluctuations. Abnormal patterns may indicate conditions like diabetes, hypoglycemia, or other metabolic issues that require further evaluation.

Reference range

Fasting: 2.6-24.9 μIU/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

How long will I need to prepare for this test?
You may need to fast for 8–12 hours before the test, but your healthcare provider will give you exact instructions. Some people may also need to avoid certain medications or activities, so follow their guidance carefully.
Will I feel any discomfort during blood collection?
You might feel a slight pinch or sting when the blood is drawn, but it usually goes away quickly. Most people experience minimal discomfort. If you’re nervous, let your provider know—they can offer tips to make it more comfortable.
Can I take my usual medications on the day of the test?
Some medications may affect insulin levels. Always check with your provider before taking anything on the day of the test, including over-the-counter drugs or supplements. They’ll let you know if you need to adjust your routine.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy