Insulin concentration in serum/plasma (24-hour profile)
This test measures your insulin levels over a 24-hour period using blood samples collected at specific times to help assess how your body produces and responds to insulin.
["A 24-hour insulin profile helps diagnose or monitor conditions like diabetes or insulin resistance."]
Key facts at a glance
- LOINC
- 27860-6
- Short code
- Insulin 2h p 75 g Glc PO SerPl-aCnc
- Synonyms
- Insulin 2h p 75 g Glc PO SerPl-aCnc
- Category
- glucose
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Ref low
- 2.6
- Ref high
- 24.9
- Fasting
- true
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- CPT
- 83525
- SNOMED
- 413618004
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
Why This Test Is Done
Your doctor may order this test if they suspect you have diabetes, prediabetes, or another condition affecting insulin production or use. It can also help evaluate how well your body responds to insulin over time, especially in people with unexplained symptoms like fatigue, weight changes, or frequent urination.
How the Test Works
Blood samples are taken at multiple times (typically every 4 hours) to measure insulin levels. The test uses a sensitive method to detect insulin in your blood, which is then analyzed to create a profile of your insulin production throughout the day.
Sample Collection Process
You’ll need to fast for a certain period before your first blood draw (usually overnight). Blood samples are collected at scheduled intervals, and you may be asked to stay at the lab or clinic for the duration. Your doctor or lab technician will explain the exact process and timing.
What to Expect Before the Test
Avoid eating or drinking anything except water for the fasting period. If you’re taking certain medications, check with your doctor first. Wear comfortable clothing and plan to stay near the lab for the full 24-hour period if required.
Reference range
Fasting: 2.6-24.9 μIU/mLN/A
No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
This test helps identify abnormal insulin levels that may indicate conditions like hypoglycemia (low blood sugar), hyperinsulinemia (high insulin), or insulin resistance. It can also rule out other metabolic disorders or help tailor treatment plans for diabetes or hormonal imbalances.
Frequently asked questions
Will I need to fast before this test?
How long will I have to stay for the test?
Can I take my usual medications before the test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy