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Insulin with 24-Hour Quantitative Measurement (Serum/Plasma)

Published: August 9, 2026Updated: August 16, 2026

This test measures your insulin levels over a 24-hour period to help assess how your body manages blood sugar and insulin production.

Quick summary

["Understanding how your insulin fluctuates can help diagnose or monitor conditions like diabetes or insulin resistance."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
47664-8
Short code
Insulin 3M p Glc SerPl-aCnc
Synonyms
Insulin 3M p Glc SerPl-aCnc
Category
glucose
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2.6
Ref high
24.9
Fasting
true
Population
adult
Sex
all
Age range
18–65 years
CPT
83525
SNOMED
413618004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if you have symptoms of insulin resistance, unexplained blood sugar fluctuations, or if you’re being evaluated for conditions like Cushing’s syndrome, acromegaly, or other hormonal imbalances. It can also help monitor how your body responds to certain medications.

How the Test Works

A blood sample is collected at multiple times over 24 hours to measure insulin levels. The results help create a profile of your insulin activity, which doctors compare to typical patterns to identify abnormalities.

What to Expect Before the Test

No special preparation is needed, but you may be asked to avoid certain medications or foods that could affect insulin levels. Follow your doctor’s instructions carefully.

Common Conditions This Test Helps Diagnose

This test can help identify conditions such as insulinoma (a tumor producing excess insulin), hyperinsulinism, or other metabolic disorders that alter how insulin behaves in your body.

Reference range

Fasting: 2.6-24.9 μIU/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

24-Hour Insulin Profile

Frequently asked questions

How often should I expect to have this test?
This test is typically done once, but your doctor may recommend repeat testing if initial results are unclear or if you have ongoing symptoms.
What do my insulin levels mean?
Insulin levels alone don’t provide a full picture—your doctor will consider them alongside other factors like blood sugar readings, symptoms, and medical history to interpret results accurately.
Can diet or exercise affect this test?
Yes, diet, exercise, and certain medications can influence insulin levels. Your doctor will provide guidance on how to prepare for the test without altering results.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy