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Serum Insulin with 24-Hour Quantification (or 'Insulin Profile')

Published: August 9, 2026Updated: August 16, 2026

This test measures your insulin levels over a 24-hour period to help assess how your body produces and uses insulin, which is important for managing blood sugar and diabetes.

Quick summary

["This test helps diagnose or monitor conditions like diabetes or insulin resistance by tracking insulin production throughout the day."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
27852-3
Short code
Insulin 5h p chal SerPl-aCnc
Synonyms
Insulin 5h p chal SerPl-aCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2.6
Ref high
24.9
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83525
SNOMED
413618004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if you have symptoms of low or high blood sugar, unexplained weight changes, frequent urination, fatigue, or if you have a family history of diabetes or metabolic disorders. It may also be used to evaluate how well your body responds to insulin therapy or to check for conditions like Cushing’s syndrome or hyperinsulinism.

What to Expect Before the Test

No special preparation is needed for this test. You may have a simple blood draw from a vein in your arm, and the sample is sent to a lab for analysis. If you’re taking insulin therapy, discuss your medication schedule with your healthcare provider before the test.

What the Test Measures

This test measures the concentration of insulin in your blood over 24 hours. Insulin is a hormone produced by your pancreas that helps regulate blood sugar levels. The test helps determine if your body is producing enough insulin or if there are fluctuations in its levels throughout the day.

How the Results Are Used

The results help your doctor understand how your body manages blood sugar, whether you have insulin resistance, or if your current treatment plan is effective. Abnormal results may indicate conditions like diabetes, hypoglycemia, or other metabolic disorders that require further evaluation.

Reference range

Fasting: 2.6-24.9 μIU/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Insulin Profile (24-hour)

Frequently asked questions

How often should I expect this test?
This test is typically done once, but your doctor may recommend repeat testing if they suspect changes in your condition, such as new symptoms, adjustments to medication, or during follow-up visits for diabetes or metabolic disorders.
What does a high or low insulin level mean?
High insulin levels may suggest insulin resistance, hyperinsulinism, or other conditions affecting blood sugar regulation. Low insulin levels could indicate diabetes, pancreatic issues, or other metabolic problems. Your doctor will interpret results in the context of your overall health and symptoms.
Can I eat or drink before this test?
You can eat and drink normally before this test unless instructed otherwise by your doctor. Since this is a 24-hour profile, fasting is not required, but your doctor may provide specific guidance based on your condition or treatment plan.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy