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Insulin (Serum or Plasma) with 24-Hour Profile

Published: August 9, 2026Updated: August 16, 2026

This test measures insulin levels over a 24-hour period to assess how your body produces and uses insulin, often used to evaluate diabetes management or insulin sensitivity.

Quick summary

["Understanding your insulin levels over time helps doctors check if your diabetes treatment is working properly."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
56942-6
Short code
Insulin 64h p chal SerPl-aCnc
Synonyms
Insulin 64h p chal SerPl-aCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2.6
Ref high
24.9
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83525
SNOMED
413618004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if you have diabetes and want to monitor how well your insulin levels fluctuate throughout the day. It can also help diagnose conditions like hypoglycemia (low blood sugar) or insulin resistance. Some people with unexplained weight changes, fatigue, or other symptoms may also need this test.

What to Expect Before the Test

You’ll typically provide a blood sample in a clinic or lab. The test involves drawing blood from a vein, usually in your arm. You may feel a small pinch, but it usually doesn’t cause much discomfort. If you’re taking insulin or other diabetes medications, let your healthcare provider know before the test.

What the Test Results Mean

Insulin levels vary naturally, especially in people with diabetes. A 24-hour profile helps track patterns, such as spikes or drops, that might indicate how well your body regulates blood sugar. Your doctor will compare these results with your symptoms and other test results to make a diagnosis or treatment plan.

Common Conditions Linked to This Test

This test is particularly useful for diagnosing or managing diabetes, including type 1 and type 2 diabetes. It may also help identify hypoglycemia, insulinoma (a rare tumor that causes high insulin levels), or other metabolic disorders affecting insulin production or function.

Reference range

Fasting: 2.6-24.9 μIU/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

What does a 24-hour insulin test involve?
This test involves collecting blood samples at multiple points over 24 hours to measure insulin levels at different times. You’ll likely provide samples at least 4–6 times, depending on your doctor’s instructions. Your doctor may ask you to keep a consistent schedule for meals, activity, and medication to ensure accurate results.
How long does it take to get results from this test?
Results from a 24-hour insulin test can take several days to a couple of weeks to process, depending on your lab and healthcare provider. Your doctor will contact you once they have the full report, which may include detailed patterns of your insulin levels throughout the day.
Can I eat or drink anything before the blood draw for this test?
Yes, you can eat and drink normally before your blood draws, unless your doctor specifies otherwise. Some tests may require fasting, but this is not the case for insulin testing. Always follow your doctor’s specific instructions to avoid interfering with the results.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy