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Insulin, Dried Blood Spot — Immunoassay, Concentration

Published: August 9, 2026Updated: August 16, 2026

Measures insulin in a dried blood spot using an immunoassay. The result is interpreted with blood glucose, symptoms, medications, and the time of collection.

Quick summary

["This test measures the amount of insulin in a small blood sample to help evaluate how the body regulates blood glucose."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
106158-9
Short code
Insulin DBS IA-aCnc
Synonyms
Insulin DBS IA-aCnc
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2.6
Ref high
24.9
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ia
CPT
83525
SNOMED
413618004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This test measures insulin, a hormone made by the pancreas that helps glucose enter cells for energy. The sample is collected as a dried blood spot and tested using an immunoassay.

Why might I need this test?

An insulin measurement may be used to investigate unexplained high or low glucose, symptoms related to blood sugar, or the effects of diabetes medicines. It is most useful when interpreted with a glucose measurement taken at the same time.

How is the test performed?

A small prick of blood is taken from the fingertip or heel, depending on age and the collection method. The blood is placed on a special card, allowed to dry, and sent to a laboratory for analysis.

How do I prepare for the test?

Fasting may or may not be required, depending on the reason for testing and the timing of any glucose or insulin treatment. Follow the instructions from your healthcare professional and record the date, time, last meal, and relevant medication doses if requested.

Reference range

Fasting: 2.6-24.9 μIU/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

An insulin result above the expected range may occur after a recent meal, with stress or infection, or when the body is resistant to insulin. A lower result may reflect the effect of insulin or diabetes medication, prolonged fasting, or less commonly, reduced insulin production. Results must be interpreted alongside glucose levels, symptoms, medications, and collection timing.

Short name

Insulin (Dried Blood Spot)

Frequently asked questions

Do I need to fast before this insulin test?
It depends on why the test was ordered. Some measurements require fasting, while others are intended to show insulin responses around a meal or glucose challenge, so follow the specific instructions provided.
Why is my insulin result reported with a glucose result?
Insulin and glucose describe related but distinct aspects of blood sugar regulation. Interpreting both together is more useful than interpreting an insulin value by itself.
Can this test diagnose diabetes?
An insulin measurement alone does not diagnose diabetes. Diagnosis usually depends on glucose testing, clinical findings, and sometimes additional laboratory evaluation.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy