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Insulin, Free-Binding, Serum/Plasma — Qualitative

Published: August 9, 2026Updated: August 16, 2026

A qualitative serum or plasma test that evaluates whether free insulin is detectable in the blood.

Quick summary

["This test reports whether free insulin is detectable and must be interpreted with glucose and other insulin results."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
44462-0
Short code
Insulin Free+Bnd SerPl Ql
Synonyms
Insulin Free+Bnd SerPl Ql
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2.6
Ref high
24.9
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83525
SNOMED
413618004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This is a qualitative laboratory test for free insulin in a serum or plasma blood sample. It generally reports whether free insulin was detected rather than providing a numeric concentration.

Why might I need this test?

A clinician may order this test when insulin results do not fit the clinical situation or when evaluating unusual blood glucose patterns. It may be considered with tests such as glucose, total insulin, and C-peptide to assess insulin production or the possible use of injected insulin.

How is the test performed?

Blood is collected and separated into serum or plasma. The laboratory uses a specialized assay to determine whether free insulin is present, with the result usually reported as detected or not detected.

How do I prepare for the test?

Follow the testing instructions provided by your clinician. The test may need to be fasting or timed closely to a meal or insulin dose, and you should not change insulin therapy unless your healthcare professional tells you to.

Reference range

Fasting: 2.6-24.9 μIU/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

A detected result means that free insulin was measurable in the sample. A not-detected result means it was below the laboratory assay's detection threshold. This result alone does not diagnose diabetes, insulin resistance, or an insulin-producing condition and should be interpreted with glucose levels, medication use, collection timing, and other insulin tests.

Short name

Free insulin binding

Frequently asked questions

What does a qualitative result mean?
It means the result is reported as detected or not detected rather than as a number. The performing laboratory may use different wording, so check the report for the specific result.
Why is the specimen listed as serum or plasma?
Either serum or plasma can be used for this type of insulin testing. The specimen type depends on the assay and the laboratory performing the test.
Can this test diagnose diabetes or insulin resistance?
No. Free insulin is interpreted alongside blood glucose, symptoms, medication history, and tests such as C-peptide or standard insulin measurements.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy