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Human Insulin Antibodies, Serum — Qualitative

Published: August 9, 2026Updated: August 16, 2026

A qualitative blood test that checks for antibodies directed against human insulin, often used in the evaluation of unexplained hypoglycemia or insulin resistance.

Quick summary

["Detecting human insulin antibodies can help explain some cases of low blood sugar, insulin resistance, or insulin allergy."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
44317-6
Short code
Insulin Human Ab Ser Ql
Synonyms
Insulin Human Ab Ser Ql
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
2.6
Ref high
24.9
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
83525
SNOMED
413618004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This test detects antibodies that bind to human insulin. These antibodies are immune-system proteins and are not the same as the insulin used to treat diabetes.

Why might I need this test?

A clinician may order this test when low blood sugar, difficulty achieving adequate blood sugar control, or an allergic reaction to insulin occurs without a clear cause. Antibodies may develop after exposure to injected human insulin.

How is the test performed?

A blood sample is collected and examined in the laboratory for insulin antibodies. The result is generally reported as detected or not detected, although some laboratories may also report a measurement that can help guide clinical interpretation.

How do I prepare for the test?

No special preparation is usually needed beyond following any instructions provided by the laboratory. Do not stop or change insulin therapy without speaking with your clinician.

Reference range

Fasting: 2.6-24.9 μIU/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Human insulin antibodies

Frequently asked questions

What does a positive result mean?
It means that insulin antibodies were detected in the blood. This can be associated with autoimmune diabetes or previous insulin exposure, but it does not by itself establish the cause of your symptoms.
Can insulin antibodies cause low blood sugar?
In some people, certain insulin antibodies can bind insulin in ways that delay its action and contribute to prolonged or recurrent low blood sugar. A clinician evaluates this possibility using the result and your medical history.
Should I stop taking insulin before this test?
No. Do not stop or change prescribed insulin unless your healthcare professional instructs you to do so. Let your clinician know which insulin products you use and when you last received a dose.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy